Reinhardt Nico P, Wassermann Marion, Härle Jessica, Romig Thomas, Kurzrock Lina, Arnold Janosch, Großmann Ernst, Mackenstedt Ute, Straubinger Reinhard K
Bacteriology and Mycology, Institute for Infectious Diseases and Zoonoses, Department of Veterinary Sciences, LMU Munich, 85764 Oberschleißheim, Germany.
Parasitology Unit, Institute of Biology, University of Hohenheim, 70599 Stuttgart, Germany.
Pathogens. 2023 Jul 8;12(7):919. doi: 10.3390/pathogens12070919.
As hosts of numerous zoonotic pathogens, the role of raccoons needs to be considered in the One Health context. Raccoons progressively expand their range as invasive alien species in Europe. This study aimed to investigate the intestinal helminth fauna of raccoons in Baden-Wuerttemberg, Germany, as no such screening had ever been conducted there. In total, we obtained 102 animals from hunters in 2019 and 2020. Intestinal helminths were retrieved using the SSCT (segmented sedimentation and counting technique) and identified morphologically and by PCR-based Sanger sequencing. Fecal samples were assessed using the ELISA PetChek IP assay (IDEXX, Germany) and flotation technique. The artificial digestion method was employed for analyzing muscle tissue. We detected species of four nematode genera (, , spp., and spp.), three cestode genera ( cf. , , and spp.), and three trematode genera (, , and spp.). spp. and spp. were not found. The invasive behavior and synanthropic habits of raccoons may increase the infection risk with these helminths in wildlife, domestic and zoo animals, and humans by serving as a connecting link. Therefore, it is crucial to initiate additional studies assessing these risks.
作为众多人畜共患病原体的宿主，浣熊的作用需要在“同一健康”背景下加以考虑。浣熊作为外来入侵物种在欧洲的活动范围正在逐步扩大。本研究旨在调查德国巴登-符腾堡州浣熊的肠道蠕虫区系，因为此前该地区从未进行过此类筛查。2019年和2020年，我们总共从猎人那里获得了102只浣熊。使用分段沉淀计数技术（SSCT）检索肠道蠕虫，并通过形态学和基于PCR的桑格测序进行鉴定。粪便样本采用ELISA PetChek IP检测法（德国IDEXX公司）和浮选技术进行评估。采用人工消化法分析肌肉组织。我们检测到了四个线虫属（、、 spp.和 spp.）、三个绦虫属（ cf. 、和 spp.）以及三个吸虫属（、和 spp.）的物种。未发现 spp.和 spp.。浣熊的入侵行为和亲近人类的习性可能通过充当连接环节，增加野生动物、家畜、动物园动物和人类感染这些蠕虫的风险。因此，开展更多评估这些风险的研究至关重要。
