从临床（泌尿道致病性大肠杆菌）和环境样本中分离出的多重耐药性大肠杆菌的特性分析。
Characterization of multidrug-resistant Escherichia coli isolated from clinical (UPEC) and environmental samples.
作者信息
Farahat Eman M, Hassuna Noha A, Hammad Adel M M, Fattah Medhat Abdel, Bakry Ibrahim A, Khairalla Ahmed S
机构信息
Minia Higher Technology Institute for Applied Health Sciences, Minia, Egypt.
Medical Microbiology and Immunology Department, Faculty of Medicine, Minia University, Minia, Egypt.
出版信息
Mol Biol Rep. 2025 Jun 3;52(1):535. doi: 10.1007/s11033-025-10618-9.
BACKGROUND
Escherichia coli (E. coli) is a significant cause of urinary tract infections (UTIs).
METHODS AND RESULTS
In this study, a total of 1,040 samples comprising treated Wastewater (57), Nile River water (400), and urine from suspected UTI patients (583) were collected between September 2019 and March 2020. E. coli was prevalent in Wastewater (84.2%), river water (63.3%), and 33.5% of urine samples. All extended-spectrum β-lactamase-producing E. coli (ESBLEC) isolates were fully resistant to penicillins and cephems. Multidrug resistance (MDR) was found in 70.7% of Wastewater and 87% of river isolates, with notable resistance to ampicillin/sulbactam, sulfamethoxazole-trimethoprim, and ciprofloxacin. Clinical isolates showed 62.5% MDR but remained susceptible primarily to ciprofloxacin and entirely to meropenem. Phylogenetic analysis revealed diverse chlorine-tolerant Uropathogenic E. coli strains, suggesting environmental spread.
CONCLUSION
These results highlight wastewater treatment plants as key reservoirs of MDR E. coli and stress the need for better surveillance and control in Egypt.
背景
大肠杆菌是尿路感染（UTIs）的重要病因。
方法与结果
在本研究中，于2019年9月至2020年3月期间共收集了1040份样本，包括经处理的废水（57份）、尼罗河水（400份）以及疑似UTI患者的尿液（583份）。大肠杆菌在废水中的检出率为84.2%，在河水中为63.3%，在33.5%的尿液样本中也有检出。所有产超广谱β-内酰胺酶的大肠杆菌（ESBLEC）分离株对青霉素和头孢菌素均完全耐药。在70.7%的废水分离株和87%的河水分离株中发现了多重耐药（MDR），对氨苄西林/舒巴坦、磺胺甲恶唑-甲氧苄啶和环丙沙星有显著耐药性。临床分离株的MDR率为62.5%，但主要对环丙沙星敏感，对美罗培南完全敏感。系统发育分析揭示了多种耐氯的尿路致病性大肠杆菌菌株，表明存在环境传播。
结论
这些结果突出了污水处理厂是MDR大肠杆菌的关键储存库，并强调了埃及加强监测和控制的必要性。
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