Toder V, Durdana A, Elrad H, Gleicher N
J Clin Lab Immunol. 1985 May;17(1):29-32.
Different parameters of natural killing were evaluated in newborn cord lymphocytes using a 51Cr-release assay, a single cell cytotoxicity technique and an immuno-fluorescence method with HNK-1 monoclonal antibody. Cord blood lymphocytes expressed a positive natural killer (NK) activity although the level of this overall NK activity was lower than that of adult control (p less than 0.05). In neonates the number of cells bearing surface HNK-1 differentiation was very low (0.7 +/- 0.3). Contrary to this finding, newborns showed only a marginal decrease in the percentage of cells capable of recognizing and binding NK-sensitive target cells. However, the killing potential of these lymphocytes was impaired more profoundly (p less than 0.05) compared to adult controls. We conclude that in the neonate two distinct populations of effector cells participate in spontaneous killing. The first group is represented by classically defined NK cells (HNK-1 positive) while the second group represents. NK-like effector cells which lack the HNK-1 antigen. It seems that in newborns the latter type of cells represents the larger factor in spontaneous killing.
采用51Cr释放试验、单细胞细胞毒性技术以及用HNK - 1单克隆抗体的免疫荧光法，对新生儿脐带血淋巴细胞的不同自然杀伤参数进行了评估。脐带血淋巴细胞表现出阳性自然杀伤（NK）活性，尽管这种总体NK活性水平低于成人对照组（p < 0.05）。在新生儿中，带有表面HNK - 1分化抗原的细胞数量非常少（0.7±0.3）。与这一发现相反，新生儿中能够识别和结合NK敏感靶细胞的细胞百分比仅略有下降。然而，与成人对照组相比，这些淋巴细胞的杀伤潜力受损更为严重（p < 0.05）。我们得出结论，在新生儿中，有两种不同的效应细胞群体参与自发杀伤。第一组由经典定义的NK细胞（HNK - 1阳性）代表，而第二组代表缺乏HNK - 1抗原的NK样效应细胞。似乎在新生儿中，后一种类型的细胞在自发杀伤中占更大因素。
