Schwartz H, Chu I, Villeneuve D C, Viau A, Benoit F M
J Toxicol Environ Health. 1985;15(5):603-7. doi: 10.1080/15287398509530689.
[14C(U)]-Labeled 1,2,3,4-tetrachlorobenzene was administered orally to squirrel monkeys. Urine was collected from these animals, pooled and analyzed for metabolites by thin-layer chromatography, high-performance liquid chromatography, and gas chromatography-mass spectroscopy. N-Acetyl-s-(2,3,4,5-tetrachlorophenyl) cysteine was shown to be the major metabolite and accounted for 85% of the radioactivity found in urine. A minor metabolite was identified as 2,3,4,5-tetrachlorophenol. This study demonstrates for the first time that an N-acetyl cysteine conjugate has been isolated and identified as metabolite of a chlorinated benzene. This pattern of chlorobenzene metabolism is significantly different from the one obtained with the rat and rabbit, where tetrachlorophenols constitute the major metabolites.
给松鼠猴经口给予[¹⁴C(U)]标记的1,2,3,4-四氯苯。收集这些动物的尿液，汇集后通过薄层色谱法、高效液相色谱法和气相色谱-质谱法分析代谢产物。结果表明，N-乙酰基-s-(2,3,4,5-四氯苯基)半胱氨酸是主要代谢产物，占尿液中放射性的85%。一种次要代谢产物被鉴定为2,3,4,5-四氯苯酚。本研究首次证明已分离并鉴定出一种N-乙酰半胱氨酸共轭物作为氯苯的代谢产物。氯苯的这种代谢模式与大鼠和兔子的代谢模式显著不同，在大鼠和兔子中，四氯苯酚是主要代谢产物。
