Yakout Amr A, Basha Maram T, Al-Raimi Doaa S, Aljadaani Abeer H, Ali Zainy Faten M
Department of Chemistry, College of Science, University of Jeddah, Jeddah, Saudi Arabia; Chemistry Department, Faculty of Science, Alexandria university, Alexandria, Egypt.
Department of Chemistry, College of Science, University of Jeddah, Jeddah, Saudi Arabia.
Int J Biol Macromol. 2025 Jul;318(Pt 1):144839. doi: 10.1016/j.ijbiomac.2025.144839. Epub 2025 Jun 1.
Water pollution has become a critical global issue, primarily due to contaminants that originate from industrial activities. The textile and plastic industries discharge synthetic dyes, which pose severe threats to ecosystems and human health. This study explores the fabrication and application of a hybrid nanocomposite (Cu/MnO/CS/GO) for the efficient removal of both anionic (Congo red, CR) and cationic (Malachite green, MG) dyes from industrial wastewater. Cu/MnO/CS/GO nanocomposite was synthesized by grafting graphene oxide (GO) onto chitosan (CS), followed by decoration with copper (Cu) and manganese dioxide (MnO) nanoparticles. The GO/CS matrix provided stable support for the uniform dispersion and stabilization of the active nanoparticles. The nanocomposite achieved remarkable dye removal efficiencies, with 99.86 % for MG at pH 8 and 99.91 % for CR at pH 5. HRTEM, SEM, EDX, FTIR, XRD, and XPS techniques were employed to confirm the successful synthesis and structural integrity of the nanocomposite. Cu/MnO/CS/GO demonstrated excellent reusability, maintaining its performance over five regeneration cycles. Under optimal conditions, effective removal of CR and MG (97.9-99.6 % ± 1.9-4.1) was achieved from three industrial wastewater samples. Moreover, cytotoxicity tests revealed that the presence of the CS/GO moiety reduced the toxicity of Cu/MnO nanoparticles in HEK293 cells. The antibacterial performance of Cu/MnO/CS/GO was assessed in comparison with Cu/MnO alone against both Gram-positive and Gram-negative bacteria, with notably improved activity observed against Staphylococcus aureus and Salmonella enterica serovar Typhi.
水污染已成为一个关键的全球问题，主要是由于源自工业活动的污染物。纺织和塑料行业排放合成染料，这对生态系统和人类健康构成严重威胁。本研究探索了一种杂化纳米复合材料（Cu/MnO/CS/GO）的制备及其用于从工业废水中高效去除阴离子染料（刚果红，CR）和阳离子染料（孔雀石绿，MG）的应用。通过将氧化石墨烯（GO）接枝到壳聚糖（CS）上，随后用铜（Cu）和二氧化锰（MnO）纳米颗粒进行修饰，合成了Cu/MnO/CS/GO纳米复合材料。GO/CS基质为活性纳米颗粒的均匀分散和稳定提供了稳定的支撑。该纳米复合材料实现了显著的染料去除效率，在pH为8时对MG的去除率为99.86%，在pH为5时对CR的去除率为99.91%。采用高分辨透射电子显微镜（HRTEM）、扫描电子显微镜（SEM）、能谱仪（EDX）、傅里叶变换红外光谱仪（FTIR）、X射线衍射仪（XRD）和X射线光电子能谱仪（XPS）技术来确认纳米复合材料的成功合成及其结构完整性。Cu/MnO/CS/GO表现出优异的可重复使用性，在五个再生循环中保持其性能。在最佳条件下，从三个工业废水样品中实现了对CR和MG的有效去除（97.9 - 99.6% ± 1.9 - 4.1）。此外，细胞毒性测试表明，CS/GO部分的存在降低了Cu/MnO纳米颗粒在人胚肾293细胞（HEK293）中的毒性。与单独的Cu/MnO相比，评估了Cu/MnO/CS/GO对革兰氏阳性菌和革兰氏阴性菌的抗菌性能，观察到对金黄色葡萄球菌和伤寒沙门氏菌血清型伤寒杆菌的活性有显著提高。
