Wang Yaping, Liu Binfeng, Zhang Ying, Feng Chengyao, Dai Weiwei
Teaching and Research Section of Clinical Nursing, Xiangya Hospital of Central South University, Changsha, Hunan, China.
Department of Orthopaedics, The Second Xiangya Hospital of Central South University, Changsha, 410011, Hunan, China.
Sci Rep. 2025 Jun 3;15(1):19473. doi: 10.1038/s41598-025-04390-w.
Osteosarcoma (OS) is a highly malignant bone tumor that primarily affects children, adolescents, and young adults. Despite significant progress in chemotherapy, surgery, and limb salvage surgery, the prognosis for patients with high-grade or metastatic OS remains poor. This study explores the role of SAC3D1 gene in OS and its potential as a prognostic marker and therapeutic target. Through analysis of public databases and experimental verification, we found that SAC3D1 is significantly upregulated in OS tissues and cell lines and is associated with poor prognosis in patients. Functional enrichment analysis suggests that SAC3D1 may promote the progression of OS by regulating cell cycle, DNA repair, and immune microenvironment pathways. In addition, the expression level of SAC3D1 is associated with the tumor immune microenvironment and immune therapy response. In vitro experiments further confirmed that knocking down SAC3D1 significantly inhibited the proliferation, migration, and invasion ability of OS cells, while its overexpression enhanced these malignant biological behaviors. In summary, SAC3D1 has important prognostic and therapeutic potential in OS, and may serve as a novel independent prognostic marker and therapeutic target for OS.
骨肉瘤（OS）是一种高度恶性的骨肿瘤，主要影响儿童、青少年和年轻成年人。尽管在化疗、手术和保肢手术方面取得了显著进展，但高级别或转移性OS患者的预后仍然很差。本研究探讨了SAC3D1基因在OS中的作用及其作为预后标志物和治疗靶点的潜力。通过对公共数据库的分析和实验验证，我们发现SAC3D1在OS组织和细胞系中显著上调，并且与患者的不良预后相关。功能富集分析表明，SAC3D1可能通过调节细胞周期、DNA修复和免疫微环境途径促进OS的进展。此外，SAC3D1的表达水平与肿瘤免疫微环境和免疫治疗反应相关。体外实验进一步证实，敲低SAC3D1可显著抑制OS细胞的增殖、迁移和侵袭能力，而其过表达则增强了这些恶性生物学行为。总之，SAC3D1在OS中具有重要的预后和治疗潜力，可能成为OS的一种新型独立预后标志物和治疗靶点。
