Imes Avery M, Pavelsky Morgan N, Badal Klodia, Kamp Derrick L, Briseño John L, Sakmar Taylor, Vogt Miranda A, Nyholm Spencer V, Heath-Heckman Elizabeth A C, Grasse Bret, Septer Alecia N, Mandel Mark J
Department of Medical Microbiology and Immunology, University of Wisconsin-Madison, Madison, WI USA.
Genetics Training Program, University of Wisconsin-Madison, Madison, WI USA.
bioRxiv. 2025 May 15:2025.01.10.632448. doi: 10.1101/2025.01.10.632448.
Functional studies of host-microbe interactions benefit from natural model systems that enable exploration of molecular mechanisms at the host-microbe interface. Bioluminescent colonize the light organ of the Hawaiian bobtail squid, , and this binary model has enabled advances in understanding host-microbe communication, colonization specificity, biofilms, intraspecific competition, and quorum sensing. The hummingbird bobtail squid, can be generationally bred and maintained in lab settings and has had multiple genes deleted by CRISPR approaches. The prospect of expanding the utility of the light organ model system by producing multigenerational host lines led us to determine the extent to which the light organ symbiosis parallels known processes in . However, the nature of the light organ, including its microbial constituency and specificity for microbial partners, have not been examined. In this report, we isolate bacteria from animals and tank water. Assays of bacterial behaviors required in the host, as well as host responses to bacterial colonization, illustrate largely parallel phenotypes in and hatchlings. This study reveals to be a valuable comparative model to complement studies in .
宿主-微生物相互作用的功能研究受益于天然模型系统，该系统能够探索宿主-微生物界面的分子机制。发光菌定殖于夏威夷短尾乌贼的发光器官，这种二元模型推动了我们在理解宿主-微生物通讯、定殖特异性、生物膜、种内竞争和群体感应方面取得进展。蜂鸟短尾乌贼可以在实验室环境中进行多代繁殖和饲养，并且已经通过CRISPR方法敲除了多个基因。通过培育多代宿主系来扩展发光器官模型系统效用的前景，促使我们确定发光器官共生与已知过程相似的程度。然而，发光器官的性质，包括其微生物组成和对微生物伙伴的特异性，尚未得到研究。在本报告中，我们从动物和养殖水箱水中分离细菌。对宿主所需细菌行为以及宿主对细菌定殖反应的分析表明，在和孵化幼体中存在很大程度上相似的表型。这项研究表明，是补充研究的一个有价值的比较模型。
