Tian Yuexun, Fernández-Santos Nadia A, Juarez Jose G, Esquivel Henry, Moller-Vasquez Andrea M, Granados-Presa María, Echeverria Adriana, Pennington Pamela, Fimbres-Macias Juan P, Roachell Walter, Lenhart Paul, Casey Theresa, Keck Molly E, Hodo Carolyn L, Downs Christopher H, Sittenauer Sarah C, Nevins Claire, Balasubramanian Sujata, Angulo Carlos, Palacios-Cardiel Carlos, Gaxiola-Robles Ramon, Zenteno-Savin Tania, Hamer Sarah A, Borden John H, Banfield Michael G, Padilla Norma, Hamer Gabriel L
Department of Entomology, Texas A&M AgriLife Research, College Station, Texas, USA.
Instituto Politecnico Nacional, Centro de Biotecnologia Genomica, Reynosa, Mexico.
bioRxiv. 2025 May 15:2025.05.09.653139. doi: 10.1101/2025.05.09.653139.
Standardized surveillance and control of kissing bugs (Hemiptera: Reduviidae: Triatominae), the insect vectors of the Chagas disease parasite , which causes Chagas disease, remains difficult. The Kissing Bug Kill Trap consists of solar powered LED lights mounted over a column of black funnels. It operates autonomously to capture, kill and preserve triatomines. We conducted experiments from 2022-2024 testing potential ways to improve trap performance, ease of deployment, and minimize cost. Thirteen prototypes evaluated in Texas, Guatemala, and Mexico captured 1,531 triatomines. In 2022-2023 we selected a six-funnel trap suspended from a single support pole with an angle bracket, and with four LED lights and a solar panel mounted above the rain-guard, as a reference trap. In 2023, traps with smaller funnels, blue funnels, and blue lights were inferior to the reference trap based on high by-catch of other arthropods and/or fewer triatomines caught per day. In 2024, traps with more or fewer than six funnels or with LED lights mounted on or below the rain guard did not outperform the reference trap. The experiments added five new triatomine species to the four already known to be caught by the Kissing Bug Kill Trap and revealed differences and similarities in phenology of dispersal flights of over a three-year period in Texas. The reference trap was selected as the pre-commercial prototype, based on its suitability for triatomine surveillance and potential for reducing the risk of infection by intercepting dispersing adult triatomines before they reach human habitats.
恰加斯病寄生虫的昆虫传播媒介锥蝽（半翅目：猎蝽科：锥蝽亚科）的标准化监测与控制仍然困难重重。锥蝽捕杀陷阱由安装在一排黑色漏斗上方的太阳能LED灯组成。它能自动运行以捕获、杀死并保存锥蝽。我们在2022年至2024年进行了实验，测试改善陷阱性能、便于部署以及降低成本的潜在方法。在得克萨斯州、危地马拉和墨西哥评估的13个原型捕获了1531只锥蝽。在2022年至2023年，我们选择了一种由单个支撑杆通过角撑架悬挂的六漏斗陷阱，在防雨罩上方安装了四个LED灯和一块太阳能板，作为参考陷阱。2023年，基于其他节肢动物的高误捕率和/或每天捕获的锥蝽数量较少，漏斗较小、蓝色漏斗和蓝色灯的陷阱不如参考陷阱。2024年，漏斗数量多于或少于六个的陷阱，或者LED灯安装在防雨罩上方或下方的陷阱，其表现均未超过参考陷阱。这些实验在已知能被锥蝽捕杀陷阱捕获的四种锥蝽基础上又增加了五个新物种，并揭示了得克萨斯州三年内锥蝽扩散飞行物候学的异同。参考陷阱因其适用于锥蝽监测以及通过在成年锥蝽到达人类栖息地之前拦截扩散的个体来降低感染风险的潜力，被选为商业前原型。
