Low-Intensity Ultrasound Stimulates TAZ in Schwann cells.

Mechanosensation, the ability of cells to detect and respond to mechanical forces by transducing them into biochemical signals, is essential for various cellular processes, including morphogenesis, development, tissue homeostasis, and response to injury. In the peripheral nervous system (PNS), Schwann cells play a critical role in nerve development, myelination, and regeneration. These cells are highly responsive to mechanical cues such as tension, compression, and shear forces, which influence their fate, proliferation, differentiation, and regenerative capacity. In this study, we demonstrate that application of Low Intensity Ultrasound (LIU) transiently increases Schwann cell proliferation. Notably, our results show that LIU selectively activates TAZ, but not YAP, both nuclear transducers of the Hippo pathway. Additionally, we show that the LIU treatment upregulates nerve growth factor (NGF) expression in both Schwann cells and sensory neurons, suggesting a role for LIU in promoting neurotrophic support. This study highlights LIU as a mechanotherapeutic tool that enhances intrinsic regenerative functions in Schwann cells, such as neurotrophic support to neurons via NGF.