Dong Xiaoling, Qin Yuehong, Zhu Zhenyu, Wen Xiaowen, Wang Shijiang, Gong Weipeng, Song Xilin, Chai Jie, Abedi-Firouzjah Razzagh, Liu Kai
Hospital ward NO.1 of Colorectal Surgery, Shandong Cancer Hospital Affiliated to Shandong First Medical University, Jinan, 250000, China.
Hospital ward NO.1, Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, 250000, China.
Iran J Biotechnol. 2025 Jan 1;23(1). doi: 10.30498/ijb.2025.484620.4010. eCollection 2025 Jan.
BACKGROUND: () infection affects nearly half of the global population and is a significant contributor to gastroduodenal conditions, including gastric carcinoma. Research on herbal extracts has highlighted their antibacterial, antioxidant, and anti-inflammatory properties, indicating potential for novel, synergistic therapeutic approaches. OBJECTIVE: To assess the synergistic antimicrobial effect of Curcumin, Licorice, and photodynamic therapy (PDT) against the infection in a rat model by evaluating the NF-κB signaling pathway. MATERIALS AND METHODS: Forty male rats (250-300 g) were divided into eight groups as follows: control, rats treated with 600 mg.kg of Curcumin and 200 mg.kg of Licorice, -infected rats, -infected rats treated with 600 mg.kg of Curcumin, -infected rats treated with 200 mg.kg of Licorice, -infected rats treated with 600 mg.kg of Curcumin and 200 mg.kg of Licorice, -infected rats treated with PDT (based on 3'-sialyllactose [3SL]-conjugated, poly-l-lysine-based photosensitizer [p3SLP]), and -infected rats treated with PDT combined with Curcumin and Licorice. The immunohistochemistry test was performed to investigate NF-κB p65 expression in gastric epithelial cells (GECs). RESULTS: The average colonization score was zero for the control and Curcumin + Licorice groups. However, the scores were 1.8, 1.0, 1.2, 0.8, 0.6, and 0.2 for the , + Curcumin, + Licorice, + Curcumin and Licorice, PDT alone treatment, and PDT + Curcumin and Licorice groups, respectively. Regarding colonization and gastric inflammation scores, the treatment effect of PDT combined with Curcumin and Licorice was significantly greater than that of the other treatments (). infection significantly increased the NF-κB expression in GECs compared to the control group (20.6%±2.1% vs. 10.1%±2.2%, P=0.035). This expression was significantly reduced in the + PDT combined with Curcumin and Licorice (8.8%±1.2%), the + Curcumin (15.3%±2.4%), and the + Licorice groups (16.4%±2.2%) compared to the only group (). The administration of Curcumin and Licorice alone did not alter baseline NF-κB expression in GECs (8.3%±1.8%, ). CONCLUSION: Our results demonstrated that combination treatment of PDT with Curcumin and Licorice may exert stronger anti-inflammatory effects by suppressing NF-κB p65 expression in GECs compared to each treatment alone. This combination could serve as a potential treatment option for future studies.
背景：（）感染影响了近一半的全球人口，是导致胃十二指肠疾病（包括胃癌）的一个重要因素。对草药提取物的研究突出了它们的抗菌、抗氧化和抗炎特性，表明了新型协同治疗方法的潜力。 目的：通过评估核因子κB（NF-κB）信号通路，评估姜黄素、甘草和光动力疗法（PDT）对大鼠模型中（）感染的协同抗菌作用。 材料与方法：40只雄性大鼠（250 - 300克）分为八组，如下：对照组、用600毫克/千克姜黄素和200毫克/千克甘草治疗的大鼠、（）感染大鼠、用600毫克/千克姜黄素治疗的（）感染大鼠、用200毫克/千克甘草治疗的（）感染大鼠、用600毫克/千克姜黄素和200毫克/千克甘草治疗的（）感染大鼠、用PDT（基于3'-唾液酸乳糖[3SL] - 共轭、聚-L-赖氨酸基光敏剂[p3SLP]）治疗的（）感染大鼠、用PDT联合姜黄素和甘草治疗的（）感染大鼠。进行免疫组织化学试验以研究胃上皮细胞（GECs）中NF-κB p65的表达。 结果：对照组和姜黄素 + 甘草组的平均（）定植分数为零。然而，（）感染组、（）感染 + 姜黄素组、（）感染 + 甘草组、（）感染 + 姜黄素和甘草组、单独PDT治疗组以及PDT + 姜黄素和甘草组的分数分别为1.8、1.0、1.2、0.8、0.6和0.2。关于（）定植和胃炎分数，PDT联合姜黄素和甘草的治疗效果明显大于其他治疗（）。与对照组相比，（）感染显著增加了GECs中NF-κB的表达（20.6%±2.1%对10.1%±2.2%，P = 0.035）。与仅（）感染组相比，（）感染 + PDT联合姜黄素和甘草组（8.8%±1.2%）、（）感染 + 姜黄素组（15.3%±2.4%）和（）感染 + 甘草组（16.4%±2.2%）中该表达显著降低。单独给予姜黄素和甘草未改变GECs中基线NF-κB表达（8.3%±1.8%）（）。 结论：我们的结果表明，与单独的每种治疗相比，PDT联合姜黄素和甘草可能通过抑制GECs中NF-κB p65的表达发挥更强的抗炎作用。这种联合可为未来研究提供一种潜在的治疗选择。
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