Elkhadragy Lobna, Castillo Caitlyn C, Li Rena, Bolt Courtni R, Rund Laurie, Thomas Faith, Lane Rachel, Gaba Ron C, Schook Lawrence B, Schachtschneider Kyle M
Department of Radiology, University of Illinois at Chicago, Chicago, IL, USA.
Department of Animal Sciences, University of Illinois at Urbana-Champaign, Urbana, IL, USA.
Int J Vet Sci Med. 2025 Jun 2;13(1):1-9. doi: 10.1080/23144599.2025.2502711. eCollection 2025.
Pigs are widely used as large animal models in biomedical research due to their physiological and anatomical similarity to humans. The Oncopig, a genetically engineered pig model harbouring Cre recombinase-inducible and transgenes, serves as a valuable model for cancer research. This study describes the generation of Oncopig breeding herds and provides a characterization of their growth rate, body size, and physiological parameters, including haematologic, biochemical, and coagulation profiles. Body weight and size were measured in male and female Oncopigs, and blood samples were collected at multiple time points from birth to one year of age. A total of 13 haematologic, 18 biochemical, and 3 coagulation parameters were analysed. While male and female Oncopigs had a comparable growth rate within the first 6 months of age, male Oncopigs exhibited a significantly higher growth rate between 6 and 12 months of age and a higher body weight at 12 months. The mean body weight at 6 months of age was 52.3 kg for male Oncopigs and 48.0 kg for female Oncopigs, while at 11 months of age it was 96.4 kg for male Oncopigs and 71.7 kg for female Oncopigs. Haematologic, biochemical, and coagulation parameters were analysed for Oncopigs under 6 months of age, over 6 months, and collectively for Oncopigs within a year of age. By providing comprehensive data on growth, haematologic, and serum biochemical parameters, this study provides a critical resource for researchers utilizing Oncopigs as large animal models for cancer research and other translational studies.
由于猪在生理和解剖学上与人类相似，因此它们在生物医学研究中被广泛用作大型动物模型。Oncopig是一种携带Cre重组酶诱导型和转基因的基因工程猪模型，是癌症研究的宝贵模型。本研究描述了Oncopig繁殖群体的产生，并对其生长速度、体型和生理参数进行了表征，包括血液学、生化和凝血指标。测量了雄性和雌性Oncopig的体重和体型，并在从出生到一岁的多个时间点采集了血样。共分析了13项血液学参数、18项生化参数和3项凝血参数。虽然雄性和雌性Oncopig在6个月龄内的生长速度相当，但雄性Oncopig在6至12个月龄之间的生长速度明显更高，12个月时体重也更高。雄性Oncopig在6个月龄时的平均体重为52.3千克，雌性为48.0千克，而在11个月龄时，雄性Oncopig为96.4千克，雌性为71.7千克。对6个月龄以下、6个月以上的Oncopig以及一岁以内的Oncopig进行了血液学、生化和凝血参数分析。通过提供关于生长、血液学和血清生化参数的全面数据，本研究为将Oncopig用作癌症研究和其他转化研究的大型动物模型的研究人员提供了重要资源。
