Chabane Meriem-Dehbia, Bedouhene Samia, Belambri Sahra-Amel, Senani Nassima, Rekeb Tinhinane, Ladj Kahina, Ksouri Riadh, Dang Pham My-Chan, El-Benna Jamel
Laboratory for Analytical Biochemistry and Biotechnology, Mouloud Mammeri University of Tizi-Ouzou, Tizi-Ouzou, Algeria.
Faculté de Médecine Xavier Bichat, Centre de Recherche sur l'Inflammation, Laboratoire d'excellence Inflamex, Université de Paris INSERM U1149, CNRS EMR 8252, 75018, Paris, France.
Inflammopharmacology. 2025 Jun 4. doi: 10.1007/s10787-025-01797-9.
Bee venom (BV) is a complex bioactive mixture known for its paradoxical ability to exert both pro-inflammatory and anti-inflammatory effects, making it a compelling modulator of inflammatory responses. In this study, we investigated the effect of BV on human neutrophil inflammatory functions in vitro and its effect on inflammation in vivo in rats. Results revealed that BV exhibited a dose-dependent inhibitory effect on formyl-Met-Leu-Phe (fMLF)-induced superoxide production by neutrophils, without affecting PMA-induced superoxide production or superoxide production by the xanthine-xanthine oxidase system. BV was not toxic to neutrophils and did not affect neutrophil adhesion, however, and surprisingly it induced myeloperoxidase release from neutrophils. Interestingly, in vivo BV effectively suppressed xylene-induced ear edema and significantly reduced carrageenan-induced peritonitis by limiting neutrophil recruitment to the peritoneum. These findings suggest that although bee venom can either inhibit or stimulate neutrophil functions in vitro, it still exerts an anti-inflammatory effect in vivo. This dual action highlights the complexity of BV's bioactive components and the importance of targeted therapeutic modulation in inflammatory conditions.
蜂毒（BV）是一种复杂的生物活性混合物，以其既能发挥促炎作用又能发挥抗炎作用的矛盾能力而闻名，使其成为炎症反应的一种引人注目的调节剂。在本研究中，我们研究了BV对体外人中性粒细胞炎症功能的影响及其对大鼠体内炎症的影响。结果显示，BV对甲酰甲硫氨酸亮氨酸苯丙氨酸（fMLF）诱导的中性粒细胞超氧化物生成具有剂量依赖性抑制作用，而不影响佛波酯（PMA）诱导的超氧化物生成或黄嘌呤-黄嘌呤氧化酶系统的超氧化物生成。然而，BV对中性粒细胞无毒，也不影响中性粒细胞黏附，令人惊讶的是，它能诱导中性粒细胞释放髓过氧化物酶。有趣的是，在体内，BV通过限制中性粒细胞向腹膜的募集，有效抑制了二甲苯诱导的耳水肿，并显著减轻了角叉菜胶诱导的腹膜炎。这些发现表明，尽管蜂毒在体外既能抑制也能刺激中性粒细胞功能，但它在体内仍发挥抗炎作用。这种双重作用凸显了BV生物活性成分的复杂性以及在炎症状态下进行靶向治疗调节的重要性。
