Vyas Achla, Gupta Revathi, Jatav Rakesh
Department of Pharmaceutical Sciences, Dr. APJ Abdul Kalam University, Indore, India.
Antiinflamm Antiallergy Agents Med Chem. 2025;24(2):114-126. doi: 10.2174/0118715230340178241008163943.
This study assessed the In-vitro Antioxidant and In-vivo Analgesics and Anti-inflammatory Activity of Leaf Extract in Rats.
Diverse pharmacological applications of plants from the Apocynaceae family are reported in the literature. ; an ornamental species belonging to the Apocynaceae family, is characterized by diverse biological activities, i.e. antioxidant, cytotoxic, thrombolytic, membrane-stabilizing, antimicrobial, and anti-proliferative effects. This species represents a perennial flora that thrives in tropical and subtropical climates.
Ultrasonication-assisted method used for plant extraction. The extracts were subjected to phytochemical screening tests, followed by total phenolic content analysis, using gallic acid as a standard. The antioxidant activity was examined by DPPH scavenging and FRAP assays. The acetic acid-induced writhing test, tail flick, and Hot plate method were used for the determination of analgesic activity. Anti-inflammatory activity by following carrageenan-induced paw edema.
ABLE treatments show analgesic effectiveness against the acid-induced pain source, tail flick, and hot plate methods at different doses of ABLE 400,200,100 mg/kg results showed respectively- 55.32, 38.67, and 22.85 (% inhibition), 89.47%, 62.57 %, 49.57%, and 100%, 92.40%, 65.33% response after 180 min of drug administration. ABLE 400 and 200 mg/kg exhibit effective results (1.43± 0.005 and 1.50± 0.008) against carrageenan- induced intoxication.
The fundamental components of antioxidants that can aid in the reduction of free radicals include phenol, flavonoids, and polyphenols. Applying DPPH and FRAP, ABLE exhibits remarkable antioxidant activity. When it comes to both centrally and peripherally acting analgesics, ABLE exhibits highly effective activity. Methanolic ABLE had a noticeable impact on paw edema caused by carrageenan. Antioxidants, alkaloids, and glycosides were present in methanolic ABLE, which allowed it to efficiently combat inflammatory mediators and the cause of pain.
Ultrasonic assistance is beneficial in isolating active metabolites in plants, with phenolic compounds exhibiting antioxidant activity. ABLE, a plant with 55% squalene, effectively combats inflammatory mediators and pain. Further investigation is needed to identify biomarkers in the plant.
本研究评估了大鼠叶片提取物的体外抗氧化、体内镇痛和抗炎活性。
文献报道了夹竹桃科植物的多种药理应用。[具体植物名称未给出]是夹竹桃科的一种观赏植物，具有多种生物活性，即抗氧化、细胞毒性、溶栓、膜稳定、抗菌和抗增殖作用。该物种是一种多年生植物，在热带和亚热带气候中生长旺盛。
采用超声辅助法进行植物提取。提取物进行植物化学筛选试验，然后以没食子酸为标准进行总酚含量分析。通过DPPH清除和FRAP试验检测抗氧化活性。采用醋酸诱导扭体试验、甩尾试验和热板法测定镇痛活性。通过角叉菜胶诱导的爪肿胀试验检测抗炎活性。
ABLE处理在不同剂量（400、200、100mg/kg）下对酸诱导的疼痛源、甩尾试验和热板法显示出镇痛效果，给药180分钟后的结果分别为-55.32%、38.67%和22.85%（抑制率），89.47%、62.57%、49.57%，以及100%、92.40%和65.33%的反应。ABLE 400和200mg/kg对角叉菜胶诱导的中毒表现出有效结果（1.43±0.005和1.50±0.008）。
有助于减少自由基的抗氧化剂的基本成分包括酚类、黄酮类和多酚类。应用DPPH和FRAP法，ABLE表现出显著的抗氧化活性。在中枢性和外周性镇痛药方面，ABLE均表现出高效活性。甲醇提取物ABLE对角叉菜胶引起的爪肿胀有显著影响。甲醇提取物ABLE中存在抗氧化剂、生物碱和糖苷，使其能够有效对抗炎症介质和疼痛原因。
超声辅助有助于分离植物中的活性代谢物，酚类化合物具有抗氧化活性。ABLE是一种含有55%角鲨烯的植物，能有效对抗炎症介质和疼痛。需要进一步研究以确定该植物中的生物标志物。
