用于近交系大鼠遗传监测的品系特异性分型抗血清的特性分析
Characterization of strain specific typing antisera for genetic monitoring of inbred strains of rats.
作者信息
Kendall C, Wagner J E
出版信息
Lab Anim Sci. 1985 Aug;35(4):364-9.
Strain-specific typing antisera (SSTA) were prepared for six inbred strains of rats by using a pooled immunization protocol. The SSTA were used in both a haemagglutination assay and a complement dependent microcytotoxicity assay to compare the usefulness of the two test systems. Both assays were simple, reliable and repeatable, and each system had distinct advantages and disadvantages. The haemagglutination assay was faster and required less specialized equipment than the microcytotoxicity assay. On the other hand, interpretation of results in the microcytotoxicity assay was easier and more objective. It was concluded that SSTA could be used with the microcytotoxicity assay and/or the haemagglutination assay to provide a simple and effective genetic monitoring method for inbred strain of rats.
通过采用混合免疫方案，为6种近交系大鼠制备了品系特异性分型抗血清（SSTA）。将SSTA用于血凝试验和补体依赖性微量细胞毒性试验，以比较这两种检测系统的实用性。两种试验均简单、可靠且可重复，每个系统都有其独特的优缺点。血凝试验比微量细胞毒性试验更快，所需的专业设备更少。另一方面，微量细胞毒性试验的结果解释更容易且更客观。得出的结论是，SSTA可与微量细胞毒性试验和/或血凝试验一起使用，为近交系大鼠提供一种简单有效的遗传监测方法。
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