Nakagawa M, Saito M
Jikken Dobutsu. 1984 Apr;33(2):187-92.
Antigenic characterization of P. pneumotropica derived from mice and rats were serologically investigated by use of hyperimmune rabbit sera against mouse strain M 1 and rat strain R 1 and absorbed sera m-1 and r-1 which were prepared by absorbing anti-M 1 serum with strain R 1 and anti-R 1 serum with strain M 1, respectively. All of 13 mouse strains employed were agglutinated with both anti-M 1 and anti-R 1 sera, but their agglutination titers with anti-M 1 serum were usually higher than those with anti-R 1 serum. Agglutination test of 13 rat strains with two antisera showed results converse to those of mouse strains. On the other hand, all the mouse strains were agglutinated with absorbed serum m-1 but not with absorbed serum r-1, while quite converse results were obtained with all the rat strains. Antigens reactive with the absorbed sera were remarkably destroyed by heating at 100 degrees C for 1 hour and treating with 1 N HCl, suggesting to be capsular antigens of bacterial cells.
利用针对小鼠菌株M 1和大鼠菌株R 1的超免疫兔血清以及分别用菌株R 1吸收抗M 1血清和用菌株M 1吸收抗R 1血清制备的吸收血清m - 1和r - 1，对源自小鼠和大鼠的嗜肺巴氏杆菌进行了血清学抗原特性研究。所使用的13株小鼠菌株均与抗M 1血清和抗R 1血清发生凝集反应，但它们与抗M 1血清的凝集效价通常高于与抗R 1血清的凝集效价。13株大鼠菌株与两种抗血清的凝集试验结果与小鼠菌株相反。另一方面，所有小鼠菌株均与吸收血清m - 1发生凝集反应，但不与吸收血清r - 1发生凝集反应，而所有大鼠菌株则得到完全相反的结果。与吸收血清反应的抗原在100℃加热1小时和用1 N盐酸处理后被显著破坏，提示为细菌细胞的荚膜抗原。
