Chen Zhuang, Ji Siyuan, Gu Chenwei, Deng Yixun, Zhao Muyun, Huang Weihong, Yang Wenming, Xu Wanzhen
School of Emergency Management, School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang, 212013, PR China.
School of Materials Science and Engineering, Jiangsu University, Zhenjiang, 212013, PR China; Changzhou Engineering and Technology Institute of Jiangsu University, Changzhou, 213164, PR China.
Talanta. 2026 Jan 1;296:128409. doi: 10.1016/j.talanta.2025.128409. Epub 2025 May 27.
Antibiotic residues in food and the environment pose a significant threat to public health, requiring advanced detection strategies. In this study, a novel D-π-A polymer nanoparticle (P-Pdots) was developed with cathodic electrochemiluminescence (ECL) performance comparable to the classical Ru(bpy)/TPrA system. The vinyl bond as a π bridge not only improved the degree of electron delocalization by extending the π-conjugation length, but effectively accelerated intramolecular charge transfer, thereby significantly enhancing the ECL signal. Transient ECL tests revealed the stability of P-Pdots radical ions and elucidated a reductive-oxidative (R-O) cathodic ECL mechanism. An ultrasensitive ECL resonance energy transfer (ECL-RET) biosensor for streptomycin detection was constructed based on the effective quenching effect of metal-organic-framework functionalized gold nanoparticles (NH-MIL-53(Al)@Au) on P-Pdots. The biosensor exhibited a wide detection range (0.5 pM-200 nM) and low detection limit (120 fM), and was successfully applied to the detection of STR residues in real samples (milk, honey and Yangtze River water). This work provides new ideas for the design of efficient R-O type ECL luminophores, and establishes a sensitive and selective sensing platform with broad applicability in environmental and food safety monitoring.
食品和环境中的抗生素残留对公众健康构成重大威胁，需要先进的检测策略。在本研究中，开发了一种新型的D-π-A聚合物纳米颗粒（P-Pdots），其阴极电化学发光（ECL）性能与经典的Ru(bpy)/TPrA体系相当。乙烯基键作为π桥，不仅通过延长π共轭长度提高了电子离域程度，而且有效地加速了分子内电荷转移，从而显著增强了ECL信号。瞬态ECL测试揭示了P-Pdots自由基离子的稳定性，并阐明了一种还原-氧化（R-O）阴极ECL机制。基于金属有机框架功能化金纳米颗粒（NH-MIL-53(Al)@Au）对P-Pdots的有效猝灭作用，构建了一种用于链霉素检测的超灵敏ECL共振能量转移（ECL-RET）生物传感器。该生物传感器具有宽检测范围（0.5 pM-200 nM）和低检测限（120 fM），并成功应用于实际样品（牛奶、蜂蜜和长江水）中链霉素残留的检测。这项工作为高效R-O型ECL发光体的设计提供了新思路，并建立了一个在环境和食品安全监测中具有广泛适用性的灵敏且选择性的传感平台。
