Song Li, Ding Yilan, Bu Shuchun, Yang Yuqin, Chai Yaqin, Fu Yingzi, Zhang Pu, Yuan Ruo
Key Laboratory of Luminescence Analysis and Molecular Sensing (Southwest University), Ministry of Education, Chongqing Engineering Laboratory of Nanomaterials & Sensor Technologies, College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.
ACS Sens. 2025 Aug 22;10(8):5802-5812. doi: 10.1021/acssensors.5c01053. Epub 2025 Jul 28.
In this study, a highly efficient electrochemiluminescent (ECL) coordination polymer, Au-Zn-DTBA, was synthesized by employing 2,2'-dithiodibenzoic acid (DTBA) as the luminescent ligand and Zn as the metal node as well as Au nanoparticles (AuNPs) as coreaction accelerators confined to DTBA via Au-S bonds for the construction of biosensors for sensitive detection of the biomarker of neurological disease, dopamine. Compared to the Zn-DTBA ECL system without a coreaction accelerator, Au-Zn-DTBA exhibits a 4-fold enhancement in ECL intensity, since AuNPs confined in Zn-DTBA could promote the coreactant KSO to produce abundant sulfate radicals SO for significantly enhancing the ECL efficiency of Zn-DTBA. As a result, an ultrasensitive ECL biosensor employing the Au-Zn-DTBA/KSO ECL system was developed for dopamine detection, achieving a low detection limit of 0.41 nM and outperforming most previously reported dopamine biosensors. This strategy demonstrates the significant potential of coreaction accelerators based on the confining effect, offering a novel approach for increasing ECL efficiency and expanding its application in clinical analysis.
在本研究中，通过使用2,2'-二硫代苯甲酸（DTBA）作为发光配体、锌作为金属节点以及金纳米颗粒（AuNPs）作为通过Au-S键限制在DTBA中的共反应促进剂，合成了一种高效的电化学发光（ECL）配位聚合物Au-Zn-DTBA，用于构建用于灵敏检测神经疾病生物标志物多巴胺的生物传感器。与没有共反应促进剂的Zn-DTBA ECL系统相比，Au-Zn-DTBA的ECL强度提高了4倍，因为限制在Zn-DTBA中的AuNPs可以促进共反应物KSO产生大量硫酸根自由基SO，从而显著提高Zn-DTBA的ECL效率。结果，开发了一种采用Au-Zn-DTBA/KSO ECL系统的超灵敏ECL生物传感器用于多巴胺检测，实现了0.41 nM的低检测限，优于大多数先前报道的多巴胺生物传感器。该策略基于限制效应展示了共反应促进剂的巨大潜力，为提高ECL效率及其在临床分析中的应用拓展提供了一种新方法。
