Zhang Jiayu, Zeng Wei, Meng Qingan, Liu Hongjun, Lu Yao, Li Shuangshuang, Peng Yongzhen
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China.
National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100124, China.
Water Res. 2025 Sep 15;284:123901. doi: 10.1016/j.watres.2025.123901. Epub 2025 May 25.
The partial denitrifying phosphorus removal coupled with anammox (PDPRA) technology holds great promise for the simultaneous removal of carbon, nitrogen, and phosphorus. However, its widespread application is hindered by the instability of nitrite accumulation and the strong dependence on volatile fatty acids (VFAs) of traditional denitrifying polyphosphate accumulating organisms (DPAOs). This study first proposes coupling partial denitrifying phosphorus removal, driven by Tetrasphaera, a novel fermentative DPAO, with anammox for efficiently treating wastewater rich in complex organic matter (e.g., amino acids and proteins). A comprehensive investigation was conducted on the synergistic metabolic mechanisms between DPAOs and anammox bacteria (AnAOB). Under a low carbon to nitrogen (C/N) ratio of 3.1 ± 0.1, Tetrasphaera utilized intracellular amino acids, particularly proline, to drive the stable and efficient nitrite accumulation, leading to a contribution of 82.64 % to total nitrogen removal through anammox. This PDPRA system ultimately achieved remarkable removal efficiency of chemical oxygen demand (COD, 84.8 ± 0.8 %), total inorganic nitrogen (TIN, 92.3 ± 0.9 %) and orthophosphate (PO-P, 92.0 ± 1.5 %). Microbial community analysis and metagenomic sequencing revealed the high abundance of Tetrasphaera and Candidatus Brocadia, with their high contribution to key functional genes (narGHI, napAB, ppk1, ppk2, hzs, hdh) confirming the co-existence and co-prosperity metabolic relationship between DPAO and AnAOB. Additionally, an even spatial distribution of Tetrasphaera and AnAOB within the biofilm was developed, further ensuring the stable and efficient removal performance. The findings of this study contribute to the broader application of the PDPRA process and provide a new approach for treating wastewater containing complex organic matter.
亚硝酸盐型厌氧氨氧化耦合部分反硝化除磷（PDPRA）技术在同时去除碳、氮和磷方面具有巨大潜力。然而，亚硝酸盐积累的不稳定性以及传统反硝化聚磷菌（DPAO）对挥发性脂肪酸（VFA）的强烈依赖阻碍了该技术的广泛应用。本研究首次提出将由新型发酵型DPAO——四球藻驱动的部分反硝化除磷与厌氧氨氧化相耦合，以有效处理富含复杂有机物（如氨基酸和蛋白质）的废水。对DPAO与厌氧氨氧化细菌（AnAOB）之间的协同代谢机制进行了全面研究。在3.1±0.1的低碳氮比（C/N）条件下，四球藻利用细胞内氨基酸（尤其是脯氨酸）驱动稳定高效的亚硝酸盐积累，通过厌氧氨氧化对总氮去除的贡献率达82.64%。该PDPRA系统最终实现了对化学需氧量（COD，84.8±0.8%）、总无机氮（TIN，92.3±0.9%）和正磷酸盐（PO-P，92.0±1.5%）的显著去除效率。微生物群落分析和宏基因组测序显示四球藻和“Candidatus Brocadia”丰度较高，它们对关键功能基因（narGHI、napAB、ppk1、ppk2、hzs、hdh）的高贡献率证实了DPAO与AnAOB之间共存共荣的代谢关系。此外，四球藻和AnAOB在生物膜内形成了均匀的空间分布，进一步确保了稳定高效的去除性能。本研究结果有助于PDPRA工艺的更广泛应用，并为处理含复杂有机物的废水提供了新方法。
