Sun Yupeng, Zhou Kaihui, Wang Yuxin, Lin Ye, Wang Weiran, Xiong Xue, Zhao Xiangru, Cui Huinan, Liang Min, Wang Qiao, Yang Shengnan, Li Li
Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, PR China.
Department of Pharmaceutical Analysis, School of Pharmacy, Hebei Medical University, Shijiazhuang 050017, PR China; CSPC ZhongQi Pharmaceutical Technology (Shijiazhuang) Co., LTD, Shijiazhuang 050035, PR China.
Food Chem. 2025 Oct 15;489:144920. doi: 10.1016/j.foodchem.2025.144920. Epub 2025 May 27.
Rapid, low-cost, and in-situ detection of illegal residues on aquatic product surfaces remains a significant challenge. Herein, we developed a versatile surface-enhanced Raman scattering (SERS) sensor by integrating instant interfacial self-assembled silver nanostar film onto flexible polytetrafluoroethylene filter membrane (AgNS/PTFE) for sensitive detection of malachite green (MG) and crystal violet (CV) in crayfish. The entire process, from room-temperature substrate preparation to detection completion, takes less than 20 min. The AgNS/PTFE flexible substrate not only provides excellent hydrophobicity for signal enhancement with minimal sample volumes (2 μL) but also ensures good uniformity, resulting in a homogeneous and dense distribution of SERS-active hot spots with superior anti-interference properties and mechanical durability. By simple "drop/wipe" operations and data visualization treatment, this method achieved detection limits as low as 10 pg/mL for MG and 7 pg/mL for CV, with in-situ analysis in spiked and actual crayfish samples, demonstrating significant potential for food safety applications.
对水产品表面非法残留进行快速、低成本的原位检测仍然是一项重大挑战。在此，我们通过将即时界面自组装银纳米星膜集成到柔性聚四氟乙烯滤膜（AgNS/PTFE）上，开发了一种通用的表面增强拉曼散射（SERS）传感器，用于灵敏检测小龙虾中的孔雀石绿（MG）和结晶紫（CV）。从室温下制备基底到完成检测的整个过程耗时不到20分钟。AgNS/PTFE柔性基底不仅具有出色的疏水性，可在最小样品体积（2μL）下增强信号，还确保了良好的均匀性，从而使SERS活性热点均匀且密集分布，具有卓越的抗干扰性能和机械耐久性。通过简单的“滴/擦”操作和数据可视化处理，该方法对MG的检测限低至10 pg/mL，对CV的检测限低至7 pg/mL，并能对加标和实际小龙虾样品进行原位分析，在食品安全应用中显示出巨大潜力。
