Yang Pan, Zeng Jiangnan, Li Liyi, Ma Ruijia, Peng Jingzhe, Zhou Wei, Fu Weiling, Wu Yongzhong, Zhang Yang
Departments of Clinical Laboratory, Radiation Oncology Center, Chongqing University Cancer Hospital, Chongqing 400030, China; School of Medicine, Chongqing University, Chongqing 400044, China.
Departments of Clinical Laboratory, Radiation Oncology Center, Chongqing University Cancer Hospital, Chongqing 400030, China.
J Infect. 2025 Jul;91(1):106526. doi: 10.1016/j.jinf.2025.106526. Epub 2025 Jun 2.
Infectious pathogens exert a profound impact on global health and socio-economic stability, positioning them as a critical focus of scientific inquiry. To safeguard public health, propel advancements in medical diagnostics, and ensure food safety, the development of efficient technologies for rapid, onsite detection of pathogens is imperative. In light of recent research breakthroughs, CRISPR/Cas-based technologies for pathogen biosafety and molecular diagnostics have emerged as particularly promising in the realm of infectious disease detection. This review succinctly introduces the working principles of CRISPR/Cas systems and thoroughly discusses the design and development of various CRISPR/Cas-based biosensors. Importantly, this paper explores the robust applications of CRISPR/Cas-assisted biosensing for emerging infectious diseases, highlighting its potential in pathogen diagnostics with features like cost-effectiveness, multiplex detection and POCT applications. Furthermore, challenges and future developments of CRISPR/Cas-based biosensors for rapid and accurate pathogen detection in specialized settings are also summarized, integrating CRISPR detection with portable POCT biosensors, nanomaterials and novel colorimetric materials. As it builds on a lot of foundational work and offers new insights and detailed reference to advance the development and application of CRISPR technologies in clinical pathogen diagnostics, opening new avenues in medical diagnostics and the prevention and control of infectious diseases.
传染性病原体对全球健康和社会经济稳定产生深远影响，使其成为科学研究的关键焦点。为保障公众健康、推动医学诊断进步并确保食品安全，开发用于快速、现场检测病原体的高效技术势在必行。鉴于最近的研究突破，基于CRISPR/Cas的病原体生物安全和分子诊断技术在传染病检测领域显得特别有前景。本综述简要介绍了CRISPR/Cas系统的工作原理，并深入讨论了各种基于CRISPR/Cas的生物传感器的设计与开发。重要的是，本文探讨了CRISPR/Cas辅助生物传感在新兴传染病中的强大应用，突出了其在病原体诊断方面的潜力，具有成本效益、多重检测和即时检测应用等特点。此外，还总结了基于CRISPR/Cas的生物传感器在特定环境中快速准确检测病原体的挑战和未来发展，将CRISPR检测与便携式即时检测生物传感器、纳米材料和新型比色材料相结合。由于它建立在大量基础工作之上，并提供了新的见解和详细参考，以推动CRISPR技术在临床病原体诊断中的开发和应用，为医学诊断以及传染病的预防和控制开辟新途径。
