Wang Yihua, Li Hui, Luo Sijian, Zhong Min, Liu Jinbo, Li Baolin
Department of Laboratory Medicine, The Affiliated Hospital of Southwest Medical University, Luzhou, China.
Molecular Diagnosis of Clinical Diseases Key Laboratory of Luzhou, Luzhou, China.
Mol Diagn Ther. 2025 Jun 18. doi: 10.1007/s40291-025-00785-7.
The CRISPR/Cas system has been extensively used in the fields of biology, food safety, and environmental monitoring. This is in part because its can to be used in combination with isothermal amplification-mediated signal amplification technology along with its extraordinary trans-cleavage ability, which has initiated a new era of biosensing applications. The popularity of functional nucleic acids has enabled aptamers to convert non-nucleic acid substances into programmable nucleic acid sequences through methods such as direct detection, lock activation, sandwich design, induction of conformations, and split aptamers. Additionally, CRISPR/Cas systems have been extended beyond nucleic acid detection to include ions, small molecules, proteins, cells, bacteria, viruses, and other non-nucleic acid-based target substances. This article provides a brief overview of the mechanisms of action of four Cas proteins, the generation of aptamers, and their combined applications. Moreover, we focus on the research progress of biosensors based on aptamer-based signal conversion combined with the CRISPR/Cas system.
CRISPR/Cas系统已在生物学、食品安全和环境监测等领域得到广泛应用。部分原因在于它可与等温扩增介导的信号放大技术结合使用，以及其非凡的反式切割能力，这开启了生物传感应用的新时代。功能性核酸的普及使适配体能够通过直接检测、锁激活、夹心设计、构象诱导和分裂适配体等方法将非核酸物质转化为可编程核酸序列。此外，CRISPR/Cas系统已从核酸检测扩展到包括离子、小分子、蛋白质、细胞、细菌、病毒和其他非核酸类目标物质。本文简要概述了四种Cas蛋白的作用机制、适配体的产生及其联合应用。此外，我们重点关注基于适配体信号转换与CRISPR/Cas系统相结合的生物传感器的研究进展。
