Frazer I H, Mackay I R, Bell J, Becker G
Liver. 1985 Jun;5(3):162-72. doi: 10.1111/j.1600-0676.1985.tb00232.x.
The mononuclear cell infiltrate in the liver was analyzed, using a panel of monoclonal antibodies (MAbs) of known specificity, in 10 patients with auto-immune chronic active hepatitis and, for contrast, in 14 with other types of chronic parenchymal liver diseases. In all cases, the mononuclear cell (MNC) infiltrate in the liver consisted mostly of T lymphocytes. Helper (Th) cells were more frequent than suppressor/cytotoxic T cells (Tsc) in the portal tracts and cirrhotic scar tissue, while Tsc were more common in the hepatic parenchyma. The number of Tsc cells in the parenchyma was greatest in patients with histologically active CAH and least in patients with quiescent cirrhosis. "Plasmacytoid" cells with the morphology of plasma cells, a hallmark of the MNC infiltrate in auto-immune CAH, were more frequent in histologically active CAH than in quiescent cirrhosis. These plasmacytoid cells were T200 + ve, and hence of bone marrow origin, but the majority expressed neither membrane nor cytoplasmic immunoglobulin nor any lineage-specific marker antigens, and hence did not fulfil criteria for B lymphocytes; however, these cells were positive for OKT10 and HLA DR. No difference was evident between the MNC infiltrate in the liver in auto-immune CAH and that in the other acute or chronic liver diseases studied, including HBV-associated CAH; hence immunohistological studies do not point to any pathological processes uniquely responsible for the pattern of hepatocyte damage seen in auto-immune CAH.
使用一组已知特异性的单克隆抗体（MAb），对10例自身免疫性慢性活动性肝炎患者的肝脏单核细胞浸润情况进行了分析，并与14例其他类型慢性实质性肝病患者进行对比。在所有病例中，肝脏中的单核细胞（MNC）浸润主要由T淋巴细胞组成。在门静脉区和肝硬化瘢痕组织中，辅助性（Th）细胞比抑制性/细胞毒性T细胞（Tsc）更常见，而Tsc在肝实质中更普遍。肝实质中Tsc细胞的数量在组织学上有活动的慢性活动性肝炎（CAH）患者中最多，在静止性肝硬化患者中最少。具有浆细胞形态的“浆细胞样”细胞是自身免疫性CAH中MNC浸润的一个标志，在组织学上有活动的CAH中比在静止性肝硬化中更常见。这些浆细胞样细胞T200呈阳性，因此起源于骨髓，但大多数既不表达膜免疫球蛋白也不表达细胞质免疫球蛋白，也不表达任何谱系特异性标记抗原，因此不符合B淋巴细胞的标准；然而，这些细胞OKT10和HLA DR呈阳性。自身免疫性CAH患者肝脏中的MNC浸润与其他研究的急性或慢性肝病（包括乙肝相关CAH）中的MNC浸润之间没有明显差异；因此，免疫组织学研究并未指出任何对自身免疫性CAH中所见肝细胞损伤模式有独特责任的病理过程。
