Jeong Seong-Hwan, Cho He-Jin, Yu Jae-Hyuk, Park Hee-Moon, Park Hee-Soo
Department of Integrative Biology, Kyungpook National University, Daegu 41566, Republic of Korea.
School of Food Science and Biotechnology, Kyungpook National University, Daegu 41566, Republic of Korea.
J Microbiol. 2025 May;63(5):e2503007. doi: 10.71150/jm.2503007. Epub 2025 May 27.
A well-conserved LAMMER kinase in yeast and filamentous fungi, is a dual-specificity kinase with multiple roles in fungal biology. In this study, we assessed the roles of LkhA in Aspergillus flavus, a toxigenic fungus that produces aflatoxin B1. lkhA deletion mutants exhibited defects in fungal growth, conidiophore development, and sclerotia formation. These mutants exhibited impaired tolerance to oxidative and cell wall stresses. Moreover, the absence of lkhA resulted in a decrease in aflatoxin B1 production. The kernel assay revealed that the lkhA deletion mutants exhibited reduced production of conidia and aflatoxin B1, implying that LkhA can affect fungal toxigenesis and pathogenicity. Taken together, these results demonstrate that LkhA is important for differentiation, mycotoxin production, and pathogenicity in A. flavus.
酵母和丝状真菌中一种保守性良好的LAMMER激酶，是一种在真菌生物学中具有多种作用的双特异性激酶。在本研究中，我们评估了LkhA在黄曲霉中的作用，黄曲霉是一种产生黄曲霉毒素B1的产毒真菌。lkhA缺失突变体在真菌生长、分生孢子梗发育和菌核形成方面表现出缺陷。这些突变体对氧化应激和细胞壁应激的耐受性受损。此外，lkhA的缺失导致黄曲霉毒素B1产量下降。籽粒试验表明，lkhA缺失突变体的分生孢子和黄曲霉毒素B1产量降低，这意味着LkhA可以影响真菌的产毒能力和致病性。综上所述，这些结果表明LkhA对黄曲霉的分化、霉菌毒素产生和致病性很重要。
