推定的组蛋白甲基转移酶DOT1调控黄曲霉中的黄曲霉毒素及致病特性。
The Putative Histone Methyltransferase DOT1 Regulates Aflatoxin and Pathogenicity Attributes in Aspergillus flavus.
作者信息
Liang Linlin, Liu Yinghang, Yang Kunlong, Lin Guinan, Xu Zhangling, Lan Huahui, Wang Xiuna, Wang Shihua
机构信息
Fujian Key Laboratory of Pathogenic Fungi and Mycotoxins, Key Laboratory of Biopesticide and Chemical Biology of Education Ministry, and School of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
出版信息
Toxins (Basel). 2017 Jul 24;9(7):232. doi: 10.3390/toxins9070232.
Lysine methyltransferases transfer methyl groups in specific lysine sites, which regulates a variety of important biological processes in eukaryotes. In this study, we characterized a novel homolog of the yeast methyltransferase DOT1 in . , and observed the roles of in . . Deletion of showed a significant decrease in conidiation, but an increase in sclerotia formation. A change in viability to multiple stresses was also found in the Δ mutant. Additionally, aflatoxin (AF) production was found severely impaired in the Δ mutant. Further analysis by qRT-PCR revealed that the transcription of AF structural genes and their regulator gene were prominently suppressed in the Δ mutant. Furthermore, our data revealed that Dot1 is important for colonizing maize seeds in . . Our research indicates that Dot1 is involved in fungal development, aflatoxin biosynthesis and fungal virulence in . , which might provide a potential target for controlling . with new strategies.
赖氨酸甲基转移酶在特定的赖氨酸位点转移甲基基团，这调节了真核生物中各种重要的生物学过程。在本研究中，我们鉴定了酿酒酵母甲基转移酶DOT1在……中的一个新同源物，并观察了其在……中的作用。缺失……显示分生孢子形成显著减少，但菌核形成增加。在Δ突变体中还发现对多种胁迫的生存能力发生了变化。此外，发现Δ突变体中的黄曲霉毒素（AF）产生严重受损。通过qRT-PCR进一步分析发现，AF结构基因及其调控基因……的转录在Δ突变体中受到显著抑制。此外，我们的数据表明Dot1对于……中玉米种子的定殖很重要。我们的研究表明Dot1参与了……中的真菌发育、黄曲霉毒素生物合成和真菌毒力，这可能为用新策略控制……提供一个潜在靶点。
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