Hui-Min Zhang, Ling-Bin Yan, Dong-Mei Yuan, Feng Liu, Xiu-Gang Cao, Dai-Yan Wang, Qin-Qin He, Jun Mu, Li-Fei Yu, Yuan Liu, Zhi-Fei Chen
The Key Laboratory of Plant Resources Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences, Guizhou University, Guiyang, China.
Research Center of Karst Ecological Environment of Guizhou University, Guiyang, China.
Front Microbiol. 2025 May 21;16:1552748. doi: 10.3389/fmicb.2025.1552748. eCollection 2025.
The "rhizosphere effect" in plants occurs within approximately 5 mm from the root surface, where microbial communities exhibit distinct species composition and structural characteristics compared to non-rhizospheric soil. Root-associated fungi play crucial roles in nutrient acquisition enhancement, stress resistance improvement, organic matter decomposition, and carbon cycle promotion. Current research shows limited understanding of the rhizospheric fungal communities in , a rare and endangered plant species.
This study conducted a comparative analysis of fungal community composition and structural differences between rhizospheric and non-rhizospheric soils. The functional roles of fungi in these distinct communities were systematically analyzed, with particular emphasis on identifying keystone fungal taxa within the rhizosphere, and explained the construction process of fungal communities.
The results showed that there was no difference in α diversity between the rhizosphere and non-rhizosphere soil fungal communities of , but the β diversity was significantly differentiated, indicating that the difference between rhizosphere and non-rhizosphere fungal communities was mainly reflected in species composition rather than species number. The two communities have common dominant phylum: Ascomycetes and Basidiomycetes, and common dominant genera: and . The functional type was mainly saprotic. Linear discriminant analysis effect size (LEfSe) analysis revealed four biomarker genera (, , , and ) enriched in the rhizospheric fungal community. Venn diagram analysis identified 169 core genera within this community, with exclusively present in the rhizosphere. Mechanistic analysis of community assembly demonstrated that stochastic processes predominantly governed the structuring of rhizospheric fungal communities.
In conclusion, this study elucidates the functional composition and assembly mechanisms of rhizospheric fungal communities in , while identifying keystone fungal taxa potentially critical to its survival. Future investigations should: Quantify the specific contribution of to ; Decipher the mechanistic linkages between these fungi and the plant's stress resistance traits; Implement plant-soil-microbe synergistic restoration strategies to enhance natural regeneration capacity of populations.
植物中的“根际效应”发生在距根表面约5毫米的范围内，与非根际土壤相比，该区域的微生物群落呈现出独特的物种组成和结构特征。与根相关的真菌在增强养分获取、提高抗逆性、分解有机物和促进碳循环方面发挥着关键作用。目前的研究表明，对于一种珍稀濒危植物物种[此处原文缺失植物名称]的根际真菌群落了解有限。
本研究对[此处原文缺失植物名称]根际和非根际土壤中的真菌群落组成及结构差异进行了比较分析。系统分析了真菌在这些不同群落中的功能作用，特别着重于识别根际内的关键真菌类群，并解释了真菌群落的构建过程。
结果表明，[此处原文缺失植物名称]根际和非根际土壤真菌群落的α多样性没有差异，但β多样性存在显著差异，这表明根际和非根际真菌群落之间的差异主要体现在物种组成而非物种数量上。这两个群落有共同的优势门：子囊菌门和担子菌门以及共同的优势属：[此处原文缺失属名]和[此处原文缺失属名]。功能类型主要为腐生型。线性判别分析效应大小（LEfSe）分析揭示了在[此处原文缺失植物名称]根际真菌群落中富集的四个生物标志物属（[此处原文缺失属名]、[此处原文缺失属名]、[此处原文缺失属名]和[此处原文缺失属名]）。维恩图分析确定了该群落内的169个核心属，其中[此处原文缺失属名]仅存在于根际。群落组装的机制分析表明，随机过程主要控制着根际真菌群落的结构。
总之，本研究阐明了[此处原文缺失植物名称]根际真菌群落中的功能组成和组装机制，同时识别了对其生存可能至关重要的关键真菌类群。未来的研究应：量化[此处原文缺失植物名称]对[此处原文缺失内容]的具体贡献；解读这些真菌与植物抗逆性状之间的机制联系；实施植物 - 土壤 - 微生物协同恢复策略以提高[此处原文缺失植物名称]种群的自然更新能力。
