Chen Xiaohong, Dou Mengke, Li Yuanhui, Su Jialan, Zhao Anjiu, Huang Xiong
College of Forestry, Sichuan Agricultural University, Chengdu, China.
Ganzi Prefecture Forestry Research Institute, Kangding, China.
Front Microbiol. 2024 Aug 29;15:1445315. doi: 10.3389/fmicb.2024.1445315. eCollection 2024.
Plant tissues harbor abundant endophytes, which are crucial for plant growth. Endophytes present in , which is enriched with medicinal components, have not been isolated and characterized yet. Here we employed meta-amplicon sequencing to identify endophytic species and examined their diversity in the leaves, petioles, roots and stems of . Our findings revealed 1,247 operational taxonomic units (OTUs) for endophytic bacteria across 210 species and 476 OTUs for endophytic fungi across 222 species. Alpha diversity analysis showed the highest endophytic bacterial diversity in roots, whereas fungal diversity was similar across the leaf, petiole and root tissues. Fungal diversity in the leaves and petioles was markedly higher than that in the stems. Furthermore, beta diversity analysis revealed similarities in the endophytic bacterial and fungal compositions between the leaves and petioles, whereas the compositions in roots and stems considerably differed from those in the leaves and petioles. At the genus level, the predominant endophytic bacteria were and , whereas the predominant endophytic fungi were and . Linear discriminant analysis effect size revealed characteristic endophytic bacterial genera specific to each tissue type and characteristic endophytic fungal genera specifically in the leaves, petioles and roots. The co-occurrence network analysis indicated that the complexity of endophyte networks was the highest in the leaves and the lowest in the stems of . Overall, this study elucidates the distribution patterns of endophytes in across various tissues, offering valuable microbial resources for the development of natural products for medicinal application.
植物组织中蕴藏着丰富的内生菌，它们对植物生长至关重要。存在于富含药用成分的[植物名称未给出]中的内生菌尚未得到分离和鉴定。在此，我们采用宏扩增子测序来鉴定内生菌种类，并检测它们在[植物名称未给出]的叶片、叶柄、根和茎中的多样性。我们的研究结果显示，在210个物种中鉴定出1247个内生细菌操作分类单元（OTU），在222个物种中鉴定出476个内生真菌OTU。α多样性分析表明，[植物名称未给出]根中的内生细菌多样性最高，而叶片、叶柄和根组织中的真菌多样性相似。叶片和叶柄中的真菌多样性明显高于茎中的。此外，β多样性分析表明，叶片和叶柄中的内生细菌和真菌组成相似，而根和茎中的组成与叶片和叶柄中的有很大差异。在属水平上，主要的内生细菌是[细菌属名称未给出]和[细菌属名称未给出]，而主要的内生真菌是[真菌属名称未给出]和[真菌属名称未给出]。线性判别分析效应大小揭示了每种组织类型特有的特征内生细菌属以及叶片、叶柄和根中特有的特征内生真菌属。共现网络分析表明，[植物名称未给出]叶片中的内生菌网络复杂性最高，茎中的最低。总体而言，本研究阐明了[植物名称未给出]中内生菌在不同组织中的分布模式，为开发药用天然产物提供了宝贵的微生物资源。
