Kirchner E, Gruhn K
Nahrung. 1985;29(7):703-9. doi: 10.1002/food.19850290714.
The differences of the non-precipitable N-quota in blood plasma, liver, intestine, and muscles after treatment with various precipitants reveal data on the height of protein precipitation in the corresponding body fraction. Organs of hens treated with picric acid (1%), trichloroacetic acid (10%), and sulphosalicylic acid were used for the protein precipitation. Because of contradictory literature data as to the most suitable concentration of sulphosalicylic acid a preliminary determination of the most favourable acid concentration was necessary. The application of a 5% solution of sulphosalicylic acid gave the highest precipitation rate depending on the analyzed organs. In the succession picric acid, trichloroacetic acid, and sulphosalicylic acid nitrogen increases in the soluble supernatant. Furthermore, dependences of the protein precipitation on the kind of the analyzed organs were indicated.
用各种沉淀剂处理后，血浆、肝脏、肠道和肌肉中不可沉淀氮含量的差异揭示了相应身体部分蛋白质沉淀高度的数据。用苦味酸（1%）、三氯乙酸（10%）和磺基水杨酸处理的母鸡器官用于蛋白质沉淀。由于关于磺基水杨酸最合适浓度的文献数据相互矛盾，因此有必要初步确定最适宜的酸浓度。根据所分析的器官，使用5%的磺基水杨酸溶液可获得最高的沉淀率。依次使用苦味酸、三氯乙酸和磺基水杨酸后，可溶性上清液中的氮含量增加。此外，还表明了蛋白质沉淀与所分析器官种类的相关性。
