Ma Haotian, Peng Jinju, Li Yang, Pan Ruixue, Long Yuner, Zhao Yining, Ding Yuexia, Ma Yi
College of Coastal Agricultural Sciences, Guangdong Ocean University, Zhanjiang, China.
PLoS One. 2025 Jun 5;20(6):e0325109. doi: 10.1371/journal.pone.0325109. eCollection 2025.
Listeria monocytogenes is a significant zoonotic pathogen capable of forming biofilms on food and other materials, representing a considerable risk to human health and animal husbandry. The use of bacteriocins as potential new antibacterial and antibiofilm reagents has attracted considerable interest. This study aimed to determine the inhibitory effects of bacteriocin PCM7-4 on L. monocytogenes biofilm formation. In this study, bacteriocin PCM7-4 of SICs (1/16 × MIC, 1/8 × MIC) significantly inhibited the formation of L. monocytogenes biofilm. Bacteriocin PCM7-4 of SICs significantly reduced the production of bacterial extracellular polysaccharides, and could decrease the bacterial motility, meanwhile, PCM7-4 significantly reduced the number and viability of bacteria within the biofilm. RT-qPCR results showed that bacteriocin PCM7-4 significantly reduced the expression of flagella, community sensing and virulence factor genes associated with biofilm formation. The results demonstrated the considerable potential of bacteriocin PCM7-4 as a therapeutic agent for the prevention and treatment of L. monocytogenes biofilms.
单核细胞增生李斯特菌是一种重要的人畜共患病原体，能够在食物和其他材料上形成生物膜，对人类健康和畜牧业构成相当大的风险。使用细菌素作为潜在的新型抗菌和抗生物膜试剂引起了广泛关注。本研究旨在确定细菌素PCM7-4对单核细胞增生李斯特菌生物膜形成的抑制作用。在本研究中，SICs的细菌素PCM7-4（1/16×MIC，1/8×MIC）显著抑制了单核细胞增生李斯特菌生物膜的形成。SICs的细菌素PCM7-4显著降低了细菌胞外多糖的产生，并能降低细菌的运动性，同时，PCM7-4显著减少了生物膜内细菌的数量和活力。RT-qPCR结果表明，细菌素PCM7-4显著降低了与生物膜形成相关的鞭毛、群体感应和毒力因子基因的表达。结果证明了细菌素PCM7-4作为预防和治疗单核细胞增生李斯特菌生物膜的治疗剂具有相当大的潜力。
