Department of Microbiology, Faculty of Medicine, Shahed University, Tehran, Iran.
Student Research Committee, Babol University of Medical Sciences, Babol, Iran.
Protein Expr Purif. 2025 Feb;226:106610. doi: 10.1016/j.pep.2024.106610. Epub 2024 Sep 19.
This research examines the impact of bacteriocin derived from Lactobacillus plantarum PTCC 1745 on the biofilm formations of A. baumannii isolates. Bacteriocin derived from L. plantarum PTCC 1745 was obtained through ammonium sulfate precipitation, cation-exchange chromatography, and reversed-phase high-performance liquid chromatography (RP-HPLC). Testing for bacteriocin susceptibility has been conducted using the broth dilution method. The anti-biofilm activity of bacteriocin was evaluated using a microtiter plate method. Quantitative real-time PCR assay evaluated bap gene expression in bacteriocin-treated cells. According to SDS-PAGE, bacteriocin from L. plantarum has a 25-kDa apparent molecular weight. The MICs of bacteriocin ranged from 30 to 120 μg/mL, while the MBCs varied between 60 and 120 μg/mL. Compared to the non-treated group, strains bacteriocin-treated isolates had 59 % less ability to form biofilm. The mean relative expression of the bap gene among the MDR A. baumannii isolates decreased by 52 % compared to the untreated control. This study demonstrated that bacteriocin derived from L. plantarum PTCC 1745 had antibacterial and antibiofilm activity against MDR A. baumannii isolates.
这项研究考察了植物乳杆菌 PTCC 1745 衍生细菌素对鲍曼不动杆菌分离株生物膜形成的影响。通过硫酸铵沉淀、阳离子交换层析和反相高效液相色谱 (RP-HPLC) 从植物乳杆菌 PTCC 1745 中获得细菌素。使用肉汤稀释法进行细菌素敏感性测试。使用微量滴定板法评估细菌素的抗生物膜活性。通过定量实时 PCR 检测细菌素处理细胞中 bap 基因的表达。根据 SDS-PAGE，植物乳杆菌的细菌素具有 25 kDa 的表观分子量。细菌素的 MIC 范围为 30 至 120 μg/mL，而 MBC 范围在 60 至 120 μg/mL 之间。与未经处理的组相比，经细菌素处理的分离株形成生物膜的能力降低了 59%。与未经处理的对照组相比，MDR 鲍曼不动杆菌分离株中 bap 基因的平均相对表达降低了 52%。本研究表明，植物乳杆菌 PTCC 1745 衍生的细菌素对 MDR 鲍曼不动杆菌分离株具有抗菌和抗生物膜活性。
