Wei Jinyuan, Zhang Xingguo, Ismael Mohamedelfatieh, Zhong Qingping
Guangdong Provincial Key Laboratory of Food Quality and Safety, College of Food Science, South China Agricultural University, Guangzhou 510642, China.
Foods. 2024 Aug 8;13(16):2495. doi: 10.3390/foods13162495.
Lactic acid bacteria (LAB) are the most common probiotics, and they present excellent inhibitory effects on pathogenic bacteria. This study aimed to explore the anti-biofilm potential of the purified active substance of , named Z102-E. The effects of Z102-E on were investigated in detail, and a transcriptomic analysis was conducted to reveal the anti-biofilm mechanism. The results indicated that the sub-MIC of Z102-E (3.2, 1.6, and 0.8 mg/mL) decreased the bacterial growth and effectively reduced the self-aggregation, surface hydrophobicity, sugar utilization, motility, biofilm formation, AI-2 signal molecule, contents of extracellular polysaccharides, and extracellular protein of . Moreover, the inverted fluorescence microscopy observation confirmed the anti-biofilm effect of Z102-E. The transcriptomic analysis indicated that 117 genes were up-regulated and 214 were down-regulated. Z102-E regulated the expressions of genes related to quorum sensing, biofilm formation, etc. These findings suggested that Z102-E has great application potential as a natural bacteriostatic agent.
乳酸菌（LAB）是最常见的益生菌，它们对病原菌具有出色的抑制作用。本研究旨在探索一种名为Z102-E的纯化活性物质的抗生物膜潜力。详细研究了Z102-E对[具体对象未提及]的影响，并进行了转录组分析以揭示其抗生物膜机制。结果表明，Z102-E的亚最小抑菌浓度（3.2、1.6和0.8 mg/mL）降低了细菌生长，并有效降低了[具体对象未提及]的自聚集、表面疏水性、糖利用、运动性、生物膜形成、AI-2信号分子、细胞外多糖含量和细胞外蛋白质含量。此外，倒置荧光显微镜观察证实了Z102-E的抗生物膜作用。转录组分析表明，117个基因上调，214个基因下调。Z102-E调节了与[具体对象未提及]群体感应、生物膜形成等相关基因的表达。这些发现表明，Z102-E作为一种天然抑菌剂具有巨大的应用潜力。
