Yaman Karadam Senem, Uzun Berrin, Çoban Berhan, Kula Atik Tuğba
Private Sada Hospital izmir, Turkey.
Turkish Republic Ministry of Health Izmir Ataturk Training and Research Hospital, Turkey.
Diagn Microbiol Infect Dis. 2025 Oct;113(2):116899. doi: 10.1016/j.diagmicrobio.2025.116899. Epub 2025 May 21.
Brucellosis is still an important public health problem worldwide. This study was aimed to investigate the seroprevalence of brucellosis in our region, Menemen, İzmir, a western Turkish province, retrospectively to determine whether variables such as sex, age and seasonal differences play a role in its seroprevalence, and to compare the performances of different serological tests used in diagnosis. Results of 5871 patient serums were included retrospectively in the study. Rose Bengal Test (RBT), Standard Tube Agglutination Test (Wright) (STAT) and Brucellacapt (BCAPT) tests were used. Analysis of the data was conducted in SPSS 25.0. While RBT was positive in 152 (2.8 %) of 5391 samples, STAT was positive in 247 (21.6 %) of the 1142 samples, and BCAPT was positive in 302 (21.3 %) of 1417 samples. Since BCAPT was accepted as the reference method, the sensitivity and specificity for the RBT were 36 % and 99 %, respectively, while the sensitivity and specificity for the STAT were 82 % and 98 %, respectively. The positivity was statistically significant in all tests. Highest intensity was in the 41-55 age group, followed by the >55 and 26-40 age groups, respectively. The positivity was statistically significantly higher in male patients than it was in female patients in all tests. When the distribution of the test positivity by months was examined, it was found to be statistically significantly higher in months between May and October. (Chi-Square X2<0.05). In conclusion, brucellosis is still an endemic problem in western Turkey. Thus, it would be appropriate to administer BCAPT and STAT primarily in the diagnosis of brucellosis.
布鲁氏菌病仍是全球重要的公共卫生问题。本研究旨在回顾性调查土耳其西部省份伊兹密尔梅内门地区布鲁氏菌病的血清流行率，以确定性别、年龄和季节差异等变量是否在其血清流行率中起作用，并比较用于诊断的不同血清学检测方法的性能。本研究回顾性纳入了5871例患者血清的检测结果。采用了玫瑰红试验（RBT）、标准试管凝集试验（Wright）（STAT）和布鲁氏菌捕获试验（BCAPT）。数据在SPSS 25.0中进行分析。在5391份样本中，RBT检测呈阳性的有152份（2.8%）；在1142份样本中，STAT检测呈阳性的有247份（21.6%）；在1417份样本中，BCAPT检测呈阳性的有302份（21.3%）。由于BCAPT被视为参考方法，RBT的敏感性和特异性分别为36%和99%，而STAT的敏感性和特异性分别为82%和98%。所有检测的阳性率均具有统计学意义。阳性强度最高的是41 - 55岁年龄组，其次分别是>55岁和26 - 40岁年龄组。在所有检测中，男性患者的阳性率在统计学上显著高于女性患者。当按月份检查检测阳性率的分布时，发现5月至10月期间的阳性率在统计学上显著更高（卡方X2<0.05）。总之，布鲁氏菌病在土耳其西部仍是一个地方病问题。因此，在布鲁氏菌病的诊断中，主要采用BCAPT和STAT进行检测是合适的。
