Bifidobacterium apoptosis induction by measuring bax and caspases on SW948 human colon cancer cell line.

BACKGROUND

Nowadays, probiotic bacteria have been considered as a factor in the prevention and treatment of cancer, especially by induction of apoptosis. The aim of study, the isolation and identification of Bifidobacterium SPP, and to investigate the effects of bacterial cell extract and cell free supernatants (CFS) was on normal cell line and colon cancer cell line through measuring caspases.

MATERIAL AND METHOD

In this study, dairy products were collected and After isolation and identification of Bifidobacterium via PCR method, the cytotoxicity effects of cell free supernatants (CFS) (B.Bifidum S2 and B.Bifidum S3) and bacterial cell extract (B.Bifidum P6 and B.Bifidum P17) on colon cancer cell line (SW948) and normal cell line (HEK-293) were evaluated using MTT assay. The effect of isolated bacterial strains on apoptotic cells was determined by measuring caspases 1, 3, 9 and Bax by using ELISA kit.

RESULT

The results showed that the cytotoxicity effect of CFS was higher than bacterial cell extract. CFS showed the highest cytotoxicity effect (about 95 %) on colon cancer cell line. Quantitative analysis of Caspase-1, -3, 9, and Bax expression demonstrated that CFS may exert anticancer effects through induced apoptosis.

CONCLUSION

The results of the present research indicate that probably B.Bifidum S2 and B.Bifidum S3 isolates may be prevent colon cancer by inducing apoptosis.