Kong Fan-Bin, Cui Ling-Ling, Zhang Bai-Zhong, Jiang Yu-Tai, Lv Yong-Po, Zhang Pei, Peng Yu-Yang, Xiong Yun-Shan, Ji Xiang, Liu Run-Qiang
College of Resources and Environment, Henan Engineering Research Center of Biological Pesticide & Fertilizer Development and Synergistic Application, Henan Institute of Science and Technology, Xinxiang, 453003, China.
Hebi Institute of Engineering and Technology, Henan Polytechnic University, Hebi, 458030, China.
Sci Rep. 2025 Jun 5;15(1):19833. doi: 10.1038/s41598-025-04684-z.
Glutathione S-transferases (GST) in pests plays a critical role in insecticide resistance. In this study, SfGSTe1 expression significant upregulation under chlorantraniliprole exposure, chlorantraniliprole susceptibility improvement after injected dsSfGSTe1 and miR-10-5p target located in the 3' UTR of SfGSTe1 conducted by luciferase reporter assays were observed in Spodoptera frugiperda (Smith). Injected miR-10-5p agomir reduced significantly SfGSTe1 abundance, together with increased chlorantraniliprole susceptibility, while injected miR-10-5p antagomir improved significantly SfGSTe1 abundance and thus decreased chlorantraniliprole susceptibility in the larvae of S. frugiperda. We revealed that the mRNA stability of SfGSTe1 by preventing its degradation that could have been induced by miR-10-5p and thus increased the resistance of S. frugiperda to chlorantraniliprole. This finding would help to study the mechanism of miRNAs in mediating insecticide susceptibility in pests.
害虫中的谷胱甘肽S-转移酶（GST）在抗药性方面起着关键作用。在本研究中，在草地贪夜蛾（史密斯）中观察到，氯虫苯甲酰胺暴露下SfGSTe1表达显著上调，注射dsSfGSTe1后氯虫苯甲酰胺敏感性提高，并且通过荧光素酶报告基因检测发现miR-10-5p的靶标位于SfGSTe1的3'UTR。注射miR-10-5p激动剂显著降低了SfGSTe1丰度，同时提高了氯虫苯甲酰胺敏感性，而注射miR-10-5p拮抗剂显著提高了SfGSTe1丰度，从而降低了草地贪夜蛾幼虫对氯虫苯甲酰胺的敏感性。我们发现miR-10-5p通过防止SfGSTe1的降解来提高其mRNA稳定性，进而增强了草地贪夜蛾对氯虫苯甲酰胺的抗性。这一发现将有助于研究miRNA介导害虫对杀虫剂敏感性的机制。
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