Institute of Industrial Crops, Shandong Academy of Agricultural Sciences, Jinan, China.
Key Laboratory of Integrated Management of Crop Diseases and Pests (Ministry of Education), College of Plant Protection, Nanjing Agricultural University, Nanjing, China.
Pest Manag Sci. 2024 Apr;80(4):1761-1770. doi: 10.1002/ps.7905. Epub 2023 Dec 9.
Cytochrome P450 monooxygenases (P450s) are recognized as a major contributor to metabolic resistance in insects to most insecticides, through gene overexpressions and protein mutations. MicroRNA (miRNA), an important post-transcriptional regulator, has been reported to promote insecticide resistance by mediating the expression of detoxification enzyme genes.
In the present study, we reported that a novel microRNA PC-5p-3991_515 was involved in the post-transcriptional regulation of CYP417A2 and mediated the triflumezopyrim susceptibility in the small brown planthopper (SBPH), Laodelphax striatellus (Fallén). The tissue expression profiles showed that CYP417A2 was highly expressed in fat body. CYP417A2 was significantly up-regulated at 12, 36, 60, 84 and 108 h after the triflumezopyrim treatment. RNA interference (RNAi) against CYP417A2 significantly increased triflumezopyrim susceptibility in SBPH. According to the prediction by miRanda and TargetScan software, three miRNAs were indicated to bind to CYP417A2. However, when oversupply of agomir, only two miRNAs, PC-3p-625_4405 and PC-5p-3991_515, significantly increased the susceptibility to triflumezopyrim and decreased CYP417A2 levels. Furthermore, PC-5p-3991_515 was confirmed to be involved in the post-transcriptional regulation of CYP417A2 by dual luciferase reporter assay. Meanwhile, PC-5p-3991_515 was co-localized with CYP417A2 in the midgut in situ hybridization.
Our findings revealed that the novel microRNA, PC-5p-3991_515, post-transcriptionally regulated CYP417A2 expression, which then mediated the triflumezopyrim susceptibility in SBPH. © 2023 Society of Chemical Industry.
细胞色素 P450 单加氧酶（P450s）被认为是昆虫对大多数杀虫剂产生代谢抗性的主要原因，这是通过基因过表达和蛋白质突变实现的。微 RNA（miRNA）是一种重要的转录后调控因子，据报道通过调节解毒酶基因的表达来促进杀虫剂抗性。
本研究报道了一种新的 microRNA PC-5p-3991_515 参与了 CYP417A2 的转录后调控，并介导了褐飞虱对三氟苯嘧啶的敏感性。组织表达谱显示 CYP417A2 在脂肪体中高表达。在三氟苯嘧啶处理后 12、36、60、84 和 108 小时，CYP417A2 显著上调。针对 CYP417A2 的 RNA 干扰（RNAi）显著增加了褐飞虱对三氟苯嘧啶的敏感性。根据 miRanda 和 TargetScan 软件的预测，有 3 个 miRNA 被指示与 CYP417A2 结合。然而，当过度供应 agomir 时，只有 2 个 miRNA，PC-3p-625_4405 和 PC-5p-3991_515，显著增加了对三氟苯嘧啶的敏感性，并降低了 CYP417A2 水平。此外，通过双荧光素酶报告基因检测证实 PC-5p-3991_515 参与了 CYP417A2 的转录后调控。同时，PC-5p-3991_515 与原位杂交中的 CYP417A2 在中肠中共同定位。
我们的研究结果表明，新型 microRNA PC-5p-3991_515 转录后调控 CYP417A2 的表达，进而介导了褐飞虱对三氟苯嘧啶的敏感性。© 2023 化学工业协会。
