Huang Dejun, Deng Xiuling, Wang Yihan, Song Qinfei, Niu Suzhen
Institute of Agro-Bioengineering, Guizhou University, Xueshi Road, Guiyang, Guizhou, China.
College of Life Sciences, Guizhou University, Jiaxiu South Road, Guiyang, Guizhou, China.
BMC Plant Biol. 2025 Jun 5;25(1):762. doi: 10.1186/s12870-025-06604-9.
Species within the Camellia sect. Theaceae of the genus Camellia, mianly distributed in Southwestern China, including Guizhou, Yunnan, and Sichuan, processes abundant wild germplasm for providing possible commercial cultivars breeding. Anthocyanins are an important functional component in tea and have been widespread attention. Two tea plants with different leaf colors: 'P113'from Camellia tachangensis F. C. Zhang and the buds and leaves are light red, 'C8' from C. sinensis (L.) Kuntze and the buds are green and the leaves purple. However, the molecular mechanism of the purple difference in bud between the C. tachangensis 'P113' and C.sinensis 'C8' has not been revealed. In this study, the anthocyanin components and the corresponding molecular mechanisms in the purple buds of C. tachangensis 'P113' and C. sinensis 'C8' were performed comparative analysis by using the transcriptome and metabolome analysis. The results showed that the relative contents of anthocyanin related metabolites were significantly different in bud between 'P113' and 'C8'. The relative content of pelargonidin, delphinidin and total proanthocyanidins in 'C8' buds was significantly higher than that in 'P113'. The expression level of the homologous genes in CHS, F3'5'H, ANS, LAR and ANR were significant difference between 'P113' and 'C8' in the anthocyanin synthesis pathway, and the expression level of homologous genes in 'C8' was higher than that in 'P113'. These differential genes have different regulatory effects on their related metabolites. The results can help us to understand the physiological metabolism of the purple difference in bud between C.tachangensis and C.sinensis, and help us to choose excellent purple tea plant strains from the different Species within the Sect. Thea.
山茶属茶组植物主要分布于中国西南部，包括贵州、云南和四川，拥有丰富的野生种质资源，可为商业栽培品种的培育提供可能。花青素是茶叶中的一种重要功能成分，已受到广泛关注。有两种叶色不同的茶树：大厂茶的‘P113’，其芽叶呈浅红色；中华茶的‘C8’，其芽为绿色，叶为紫色。然而，大厂茶‘P113’和中华茶‘C8’芽紫色差异的分子机制尚未揭示。本研究通过转录组和代谢组分析，对大厂茶‘P113’和中华茶‘C8’紫色芽中的花青素成分及相应分子机制进行了比较分析。结果表明，‘P113’和‘C8’芽中花青素相关代谢物的相对含量存在显著差异。‘C8’芽中pelargonidin、delphinidin和总原花青素的相对含量显著高于‘P113’。花青素合成途径中CHS、F3'5'H、ANS、LAR和ANR同源基因的表达水平在‘P113’和‘C8’之间存在显著差异，‘C8’中同源基因的表达水平高于‘P113’。这些差异基因对其相关代谢物具有不同的调控作用。该结果有助于我们了解大厂茶和中华茶芽紫色差异的生理代谢，有助于我们从茶组不同物种中筛选优良的紫茶植株品系。
