Hobi R, Kuenzle C C
Neurosci Lett. 1985 Aug 5;58(3):311-4. doi: 10.1016/0304-3940(85)90072-2.
To test for metabolic deoxyribonucleic acid (DNA) turnover in differentiating neurons, [methyl-3H]thymidine was injected into the lateral cerebral ventricles of newly born rats, and after 6, 24 and 96 h, neuronal nuclei were prepared from the immature cerebral cortex. Enzymatic treatment converted virtually all of the DNA into soluble deoxynucleosides which were fractionated by high-performance liquid chromatography for determination of specific activity. The specific activity of thymidine was found to decline rapidly with time. The rate of this loss correlated with the radioactivity initially incorporated into the DNA. This suggested that DNA was being replaced by DNA repair as a consequence of radiation damage, rather than by spontaneous metabolic DNA turnover.
为了检测分化神经元中的代谢脱氧核糖核酸（DNA）周转率，将[甲基-³H]胸腺嘧啶核苷注入新生大鼠的侧脑室，在6小时、24小时和96小时后，从未成熟的大脑皮层制备神经元细胞核。酶处理将几乎所有的DNA转化为可溶性脱氧核苷，通过高效液相色谱法对其进行分级分离以测定比活性。发现胸腺嘧啶核苷的比活性随时间迅速下降。这种损失的速率与最初掺入DNA中的放射性相关。这表明DNA是由于辐射损伤而通过DNA修复被取代，而不是通过自发的代谢DNA周转。
