鸭疫里默氏杆菌伴侣蛋白DnaK在抗生素抗性和致病性中的功能表征

Functional characterization of the chaperone DnaK of Riemerella anatipestifer in antibiotic resistance and pathogenicity.

作者信息

Guo Fang, Shi Chunfeng, Huang Li, Yao Yizhou, Qi Yingji, Zhu Dekang, Wang Mingshu, Jia Renyong, Chen Shun, Zhao Xinxin, Wu Ying, Zhang Shaqiu, Zhang Ling, Yu Yanling, Götz Friedrich, Liu Mafeng, Cheng Anchun

机构信息

Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu 611130, China; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu 611130, China; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu 611130, China; Research Center of Avian Disease, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu 611130, China.

Microbial Genetics, Interfaculty Institute of Microbiology and Infection Medicine Tübingen (IMIT), University of Tübingen, Tübingen 72076, Germany.

出版信息

Poult Sci. 2025 May 28;104(9):105362. doi: 10.1016/j.psj.2025.105362.

DOI:10.1016/j.psj.2025.105362

PMID:40479766

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12173114/

Abstract

Antibiotic resistance has become a global public health problem, which is closely related to humans, animals and the environment. Riemerella anatipestifer (R. anatipestifer, RA) is a Gram-negative, multi-resistant bacterium that infects ducks and other birds. However, the mechanisms underlying R. anatipestifer's resistance to antibiotics aren't fully to be understood. Here, we show that the R. anatipestifer dnaK-deficient strain (∆dnaK) is more sensitive than the wild type to various tested antibacterial agents. DnaK is important for alleviating oxidative stress damage, which has been shown to be necessary for efficient scavenging of reactive oxygen species (ROS) induced by bactericidal antibiotics. Furthermore, it is also essential for maintaining normal cell morphology and membrane permeability. Finally, the chaperone DnaK is also critical for the pathogenicity of R. anatipestifer, since it is required for the heat stress resistance inside ducks. Taken together, this study is important to provide strategies against the emergence of antimicrobial resistance and R. anatipestifer infection.

摘要

抗生素耐药性已成为一个全球性的公共卫生问题，这与人类、动物和环境密切相关。鸭疫里默氏菌（Riemerella anatipestifer，RA）是一种革兰氏阴性、多重耐药细菌，可感染鸭和其他禽类。然而，鸭疫里默氏菌对抗生素耐药的潜在机制尚未完全明确。在此，我们发现鸭疫里默氏菌dnaK基因缺失菌株（∆dnaK）对各种测试抗菌剂的敏感性高于野生型。DnaK对于减轻氧化应激损伤很重要，氧化应激损伤已被证明是有效清除杀菌抗生素诱导的活性氧（ROS）所必需的。此外，它对于维持正常的细胞形态和膜通透性也至关重要。最后，伴侣蛋白DnaK对鸭疫里默氏菌的致病性也很关键，因为它是鸭体内耐热应激所必需的。综上所述，本研究对于提供对抗抗菌药物耐药性出现和鸭疫里默氏菌感染的策略具有重要意义。