Wang Bangfeng, Liu Yangcheng, Li Shunji, Zhang Ying, Chen Dongjuan, Li Mingxuan, Liu Zetai, Chen Peng, Li Yiwei, Feng Xiaojun, Liu Bi-Feng, Lin Wei
Institute of Science and Technology for Brain-Inspired Intelligence and Research Institute of Intelligent Complex Systems, Fudan University, Shanghai, 200433, China.
The Key Laboratory for Biomedical Photonics of MOE at Wuhan National Laboratory for Optoelectronics - Hubei Bioinformatics & Molecular Imaging Key Laboratory, Systems Biology Theme, Department of Biomedical Engineering, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, 430074, China.
Biosens Bioelectron. 2025 Nov 1;287:117653. doi: 10.1016/j.bios.2025.117653. Epub 2025 Jun 2.
The global prevalence of Human Papillomavirus (HPV) underscores an urgent need for solutions of accessible and rapid diagnostics. Although microfluidic chips have paved a promising pathway for achieving point-of-care-testing (POCT), the reliance on bulky and high-cost pumps has restricted their applicability in decentralized settings. Here, we present "Music-Box", a system leveraging a novel actuation mechanism based on the asymmetric motion of the speaker to achieve precise microfluidic control. By eliminating the need for fixed unidirectional structures, this system enables bidirectional fluid manipulation with high precision. Coupled with a smartphone, Music-Box offers a sample-to-result workflow for HPV nucleic acid detection. Clinical verification using 50 hospital-sourced samples reveals that the device has a 97.3 % sensitivity and 100 % specificity for HPV detection. By integrating portability, affordability, and precision, Music-Box redefines the method of POCT, enabling scalable diagnostics not only under resource-limited settings but also adaptable to scenarios of various infectious diseases.
人乳头瘤病毒（HPV）在全球的流行凸显了对便捷快速诊断解决方案的迫切需求。尽管微流控芯片为实现即时检测（POCT）开辟了一条充满希望的途径，但对笨重且昂贵的泵的依赖限制了它们在分散环境中的适用性。在此，我们展示了“音乐盒”，这是一种利用基于扬声器不对称运动的新型驱动机制来实现精确微流控控制的系统。通过无需固定单向结构，该系统能够实现高精度的双向流体操控。与智能手机相结合，“音乐盒”提供了用于HPV核酸检测的从样本到结果的工作流程。使用50份来自医院的样本进行的临床验证表明，该设备对HPV检测的灵敏度为97.3%，特异性为100%。通过整合便携性、可承受性和精确性，“音乐盒”重新定义了即时检测方法，不仅能够在资源有限的环境中实现可扩展诊断，还能适应各种传染病的检测场景。
