Augustine P C
Poult Sci. 1985 Sep;64(9):1644-8. doi: 10.3382/ps.0641644.
Electrophoretic separation of serum proteins of Eimeria-infected turkeys showed five major fractions with significant decreases in albumin levels and significant increases in the alpha 2- and gamma-globulins as compared with controls. The alpha 1- and beta-globulin levels were similar to those of the controls. Significant differences between infected turkeys and uninfected pair-fed controls suggested that protein changes were not solely due to the anorexia associated with Eimeria infections. The increase in alpha 2-globulins was not due to an increase in ceruloplasmin. Indirect fluorescent antibody tests using serum from infected poults as an antibody source were positive, indicating that at least part of the increase in the gamma-globulin fraction was due to the production of immunoglobulins. Electrophoretic separation of plasma lipoproteins showed three to four major bands with a sharp decrease in the portomicrons among infected poults. There was no increase in the pre-beta-lipoproteins (which have the same electrophoretic mobility as the alpha 2-globulins), indicating that the increase in alpha 2-globulins was not due to an increase in the lipoprotein part of this fraction.
感染艾美耳球虫的火鸡血清蛋白的电泳分离显示，与对照组相比，有五个主要组分，白蛋白水平显著降低，α2球蛋白和γ球蛋白显著升高。α1球蛋白和β球蛋白水平与对照组相似。感染火鸡与未感染的配对饲养对照组之间的显著差异表明，蛋白质变化并非仅由艾美耳球虫感染相关的厌食症引起。α2球蛋白的增加并非由于铜蓝蛋白增加。使用感染雏鸡的血清作为抗体来源进行的间接荧光抗体试验呈阳性，表明γ球蛋白组分的增加至少部分是由于免疫球蛋白的产生。感染雏鸡血浆脂蛋白的电泳分离显示有三到四条主要条带，其中前β脂蛋白显著减少。前β脂蛋白（其电泳迁移率与α2球蛋白相同）没有增加，表明α2球蛋白的增加并非由于该组分中脂蛋白部分的增加。
