Park Eun-Mee, Kim Seheon, Lim Seoyoen, Rahimizadeh Parastou, Jeon Hyeyoon, Lim Hyung Jin, Lee Sungjin, Song Yong Bhum
Division of Research Center, Scripps Korea Antibody Institute, Chuncheon, 24341, Republic of Korea.
Appl Microbiol Biotechnol. 2025 Jun 7;109(1):139. doi: 10.1007/s00253-025-13530-1.
Severe fever with thrombocytopenia syndrome (SFTS) is an emerging viral infectious disease caused by a novel Bandavirus in the family Phenuiviridae. The SFTS virus (SFTSV) is transmitted to various hosts, including humans, through tick bites, leading to high fever, thrombocytopenia, and leukopenia, with a high case fatality rate (up to 30%) due to multiple organ dysfunction. Therefore, early diagnosis is crucial for effective treatment and preventing disease transmission. In this study, we aimed to develop and characterize monoclonal antibodies targeting the SFTSV nucleocapsid protein (NP). We generated recombinant NP to screen antibodies against SFTSV. Using phage display technology, we identified candidate single-chain variable fragment (scFv) sequences capable of detecting SFTSV NP. Five human IgG antibodies and six chimeric mouse antibodies exhibited strong binding ability to the recombinant NP. Furthermore, their specificity and selectivity were evaluated against NPs from different subtypes and other viral species. A sandwich enzyme-linked immunosorbent assay (ELISA) was performed to determine optimal antibody pairings for SFTSV detection. The mP01A05/hP01C09, mP01A05/hP01B10, and mP02E04/hP01A05 antibody pairs demonstrated high efficacy in diagnosing SFTSV infections. These findings provide valuable antibody resources and establish an effective platform for the diagnosis of SFTS. KEY POINTS: • Monoclonal antibodies targeting SFTSV NP were developed using phage display technology. • Candidate antibodies showed strong binding ability and high specificity to SFTSV NP. • Optimized antibody pairs enabled effective SFTSV detection via sandwich ELISA.
严重发热伴血小板减少综合征（SFTS）是由布尼亚病毒科一种新型本扬病毒引起的新发病毒性传染病。SFTS病毒（SFTSV）通过蜱叮咬传播给包括人类在内的各种宿主，导致高热、血小板减少和白细胞减少，由于多器官功能障碍，病死率很高（高达30%）。因此，早期诊断对于有效治疗和预防疾病传播至关重要。在本研究中，我们旨在开发和鉴定靶向SFTSV核衣壳蛋白（NP）的单克隆抗体。我们制备了重组NP以筛选抗SFTSV的抗体。利用噬菌体展示技术，我们鉴定出能够检测SFTSV NP的候选单链可变片段（scFv）序列。五种人IgG抗体和六种嵌合鼠抗体对重组NP表现出强结合能力。此外，还针对来自不同亚型和其他病毒种类的NP评估了它们的特异性和选择性。进行了夹心酶联免疫吸附测定（ELISA）以确定用于SFTSV检测的最佳抗体配对。mP01A05/hP01C09、mP01A05/hP01B10和mP02E04/hP01A05抗体配对在诊断SFTSV感染方面显示出高效能。这些发现提供了有价值的抗体资源，并建立了一个有效的SFTS诊断平台。要点：•利用噬菌体展示技术开发了靶向SFTSV NP的单克隆抗体。•候选抗体对SFTSV NP表现出强结合能力和高特异性。•优化的抗体配对通过夹心ELISA能够有效检测SFTSV。
