Miraglia T, Pinto G, Ledoux L
Acta Histochem. 1977;58(1):156-62. doi: 10.1016/S0065-1281(77)80117-7.
The liver cells of 8 rabbits fed for 12 to 16 weeks with a cholesterol diet showed a decrease of RNA and a stronger reactivity for acid phosphatase and beta-glucuronidase. The non-specific esterase showed a weaker reactivity in the cells with a larger amount of cholesterol and a stronger reaction in the cells with a smaller quantity of this substance. Several enzymes such as: diaphorases, those of the pentose shunt and respiratory cycle as well as those related to the metabolism of lipids, proteins and mucopolysaccharides showed a unconspicuous reactivity. The glycogen was found in a lesser amont in 6 out of 8 rabbits but with a higher concentration in 2 of them. The reactivity of UDPG-GT, active phosphorylase, F-1,6-PA, F-1,6-P Ald, LDH and ADH was stronger in the last 2 animals but weaker in the 6 others. In this 8 rabbit group the total phosphorylase and G-6-PA showed respectively always a stronger and a weaker reactivity than their controls. In the liver of 2 cholesterol-fed for 20 weeks rabbits it was observed a centrolobular fibrosis, a smaller amount of RNA and glycogen as well as a global decrease of the enzymatic reactivity.
8只兔子用胆固醇饮食喂养12至16周后，其肝细胞显示RNA减少，酸性磷酸酶和β-葡萄糖醛酸酶的反应性增强。非特异性酯酶在胆固醇含量较高的细胞中反应性较弱，而在该物质含量较低的细胞中反应较强。几种酶，如：黄递酶、戊糖旁路和呼吸循环的酶以及与脂质、蛋白质和粘多糖代谢相关的酶，其反应性不明显。8只兔子中有6只肝脏中的糖原含量较少，但其中2只浓度较高。在最后2只动物中，UDPG-GT、活性磷酸化酶、F-1,6-PA、F-1,6-P醛缩酶、LDH和ADH的反应性较强，而在另外6只中较弱。在这8只兔子的组中，总磷酸化酶和G-6-PA的反应性分别始终比其对照组更强和更弱。在2只用胆固醇喂养20周的兔子的肝脏中，观察到中央小叶纤维化、RNA和糖原含量较少以及酶反应性整体下降。
