Miraglia T, Siqueira L A, Gorini D, Pinto G
Acta Anat (Basel). 1975;91(1):57-70.
The histochemistry of the hepatic parenchymal cells was studied in four Callithrix jacchus. A large amount of glycogen was noted throughout the lobules while the UDPG-GT and the phosphorylases were found unevenly distributed by the hepatic strands with different degrees of reactivity. Near the central vein one of the livers showed PAS-positive nuclear corpuscles that were more conspicuous in the hepatic cells with a larger amount of cytoplasmic glycogen and weaker UDPG-GT and phosphorylase reactivities. G-6PA (in a larger amount) and LDH (in a moderate amount) were found evenly distributed in the hepatic strands. F-1-6PA was seen sometimes with a stronger reactivity at the peripheral part of the lobules. The enzymes of the pentose shunt (G-6PDH, 6-PGDH and NADPH-2-TR) reacted strongly and as a rule evenly distributed near the hepatic lobules. Occasionally they reacted more intensely in the row of hepatic cells disposed just around the central vein. Cytochrome oxidase showed a very faint reaction. Cis-aconitase and ICDH were weak or moderate. NADH-2-TR more than SDH more than MDH were seen frequently diffused near the hepatic strands. SDH and MDH in some instances showed a stronger reactivity in the row or group of hepatic cells around the central vein. ATPase at pH 6.3 was negative in the marmoset liver; ATPase at pH 7.4 was mainly found in the wall of the portal area vessels; ATPase at pH 8.5 showed a stronger reactivity in the cytoplasm of the hepatic cells and ATPase at pH 9.4 was more abundant in the bile capillaries. The reactivity of the lipid metabolism enzymes was moderate with regard to alpha-GPDH or negligible with regard to beta-OHBDH. Acid phosphatase showed a stronger reaction, but almost limited to the Kupffer cells. The hepatic cells showed only a moderate amount of RNA. Some enzymes of the protein metabolism, such as GDH and leucine aminopeptidase showed a stronger reactivity while some others, such as alanyl aminopeptidase and MAO, were seen diffused near the hepatic lobules in a small amount. Enzymes of the mucopolysaccharide metabolism were not found at all (beta-glucuronidase) or showed only a weak reactivity, such as xylitol dehydrogenase.
对4只普通狨猴的肝实质细胞进行了组织化学研究。整个肝小叶内可见大量糖原，而尿苷二磷酸葡萄糖 - 葡糖基转移酶（UDPG - GT）和磷酸化酶在肝索中分布不均，反应程度不同。在其中一只肝脏的中央静脉附近，可见PAS阳性核小体，在细胞质糖原含量较多、UDPG - GT和磷酸化酶反应较弱的肝细胞中更为明显。6 - 磷酸葡萄糖酸（G - 6PA，含量较多）和乳酸脱氢酶（LDH，含量中等）在肝索中分布均匀。有时在肝小叶周边部分可见果糖 - 1,6 - 二磷酸（F - 1 - 6PA）反应较强。戊糖旁路的酶（6 - 磷酸葡萄糖脱氢酶（G - 6PDH）、6 - 磷酸葡萄糖酸脱氢酶（6 - PGDH）和还原型辅酶Ⅱ - 2 - 三磷酸吡啶核苷酸（NADPH - 2 - TR））反应强烈，通常在肝小叶附近分布均匀。偶尔在中央静脉周围的一排肝细胞中反应更强烈。细胞色素氧化酶反应非常微弱。顺乌头酸酶和异柠檬酸脱氢酶反应较弱或中等。烟酰胺腺嘌呤二核苷酸 - 2 - 三磷酸吡啶核苷酸（NADH - 2 - TR）的扩散现象比琥珀酸脱氢酶（SDH）和苹果酸脱氢酶（MDH）更常见，在肝索附近。在某些情况下，SDH和MDH在中央静脉周围的一排或一组肝细胞中反应较强。普通狨猴肝脏中pH 6.3的三磷酸腺苷酶（ATPase）呈阴性；pH 7.4的ATPase主要存在于门管区血管壁；pH 8.5的ATPase在肝细胞细胞质中反应较强，pH 9.4的ATPase在胆小管中含量更丰富。脂类代谢酶中，α - 甘油磷酸脱氢酶（alpha - GPDH）反应中等，β - 羟丁酸脱氢酶（beta - OHBDH）反应可忽略不计。酸性磷酸酶反应较强，但几乎仅限于库普弗细胞。肝细胞仅含有中等量的RNA。蛋白质代谢的一些酶，如谷氨酸脱氢酶（GDH）和亮氨酸氨肽酶反应较强，而其他一些酶，如丙氨酰氨肽酶和单胺氧化酶（MAO），在肝小叶附近少量扩散。未发现黏多糖代谢酶（β - 葡萄糖醛酸酶）或仅显示微弱反应，如木糖醇脱氢酶。
