Wan Wenjie, Grossart Hans-Peter, Xiong Xiang, He Donglan, Yang Yuyi
Key Laboratory of Lake and Watershed Science for Water Security, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China; Danjiangkou Wetland Ecosystem Field Scientific Observation and Research Station, Chinese Academy of Sciences & Hubei Province, Wuhan, 430074, China; Hubei Key Laboratory of Wetland Evolution & Ecological Restoration, Wuhan Botanical Garden, Chinese Academy of Sciences, Wuhan, 430074, China.
Leibniz-Institute of Freshwater Ecology and Inland Fisheries (IGB), Neuglobsow, 16775, Germany; Institute of Biochemistry and Biology, Potsdam University, Potsdam, 14469, Germany.
Environ Res. 2025 Oct 1;282:122078. doi: 10.1016/j.envres.2025.122078. Epub 2025 Jun 5.
Microbial biomass phosphorus (P) is an important P pool and its turnover can supplement the P pool in soil solution promoting plant productivity, and ubiquitously distributed alkaline phosphatase-encoding bacteria (PEB) containing the phoD gene mediate organic P mineralization. Yet, linkage between soil PEB and P remains poorly understood, particularly in changing environments. Molecular and statistical tools were adopted in both environmental change simulation experiments and a field investigation to unveil the linkage between soil P and PEB. Simulation experiments revealed that bacterial rather than fungal P responded significantly to three types of environmental change (i.e., rainfall, acidification/alkalization, and warming), whereby higher P content and P-cycling gene abundance were found under conditions of low environmental changes than of pronounced environmental changes. P content was significantly positively correlated with phoD gene abundance in both simulated experiments and the field survey, thereby, specific bacterial genera (e.g., Stenotrophomonas) were notably correlated with P. According to abundance and presence-absence identification of rare vs. abundant PEB, we found that rare rather than abundant PEB displayed a higher community diversity, closer phylogenetic clustering, stronger environmental restriction, and broader environmental breadth. In contrast, abundant PEB showed stronger species replacement and phylogenetic signals compared to rare ones. Rare and abundant PEB constituted stochasticity-governed community assemblages, and stochastic processes affected community assemblage of abundant PEB more than rare PEB. Our study extends our knowledge on a potential contributor of phoD gene to soil P, and our findings reveal how rare and abundant PEB remain diverse and respond to changed environments.
微生物生物量磷是一个重要的磷库，其周转可以补充土壤溶液中的磷库，促进植物生产力，而含有phoD基因的普遍分布的碱性磷酸酶编码细菌介导有机磷矿化。然而，土壤中碱性磷酸酶编码细菌与磷之间的联系仍知之甚少，尤其是在不断变化的环境中。在环境变化模拟实验和实地调查中均采用了分子和统计工具，以揭示土壤磷与碱性磷酸酶编码细菌之间的联系。模拟实验表明，细菌而非真菌的磷对三种环境变化（即降雨、酸化/碱化和变暖）有显著响应，在环境变化较小的条件下比环境变化显著的条件下发现了更高的磷含量和磷循环基因丰度。在模拟实验和实地调查中，磷含量均与phoD基因丰度显著正相关，因此，特定的细菌属（如嗜麦芽窄食单胞菌）与磷显著相关。根据稀有与常见碱性磷酸酶编码细菌的丰度和有无鉴定，我们发现稀有而非常见的碱性磷酸酶编码细菌表现出更高的群落多样性、更紧密的系统发育聚类、更强的环境限制和更广泛的环境广度。相比之下，常见的碱性磷酸酶编码细菌比稀有细菌表现出更强的物种替代和系统发育信号。稀有和常见的碱性磷酸酶编码细菌构成了受随机性控制的群落组合，随机过程对常见碱性磷酸酶编码细菌群落组合的影响大于稀有细菌。我们的研究扩展了我们对phoD基因对土壤磷潜在贡献者的认识，我们的发现揭示了稀有和常见的碱性磷酸酶编码细菌如何保持多样性并对变化的环境做出反应。
