Song Jian-Heng, Huang Jing-Yu, Guo Xin-Yu, Song Jia-Jie, Huang Yu-Ming, Wang Chen-Yu, Feng Yong-Zhong, Ren Guang-Xin, Wang Xing
Shaanxi Engineering Research Center of Circular Agriculture, Yangling 712100, China.
College of Agriculture, Northwest A&F University, Yangling 712100, China.
Huan Jing Ke Xue. 2025 Jun 8;46(6):3957-3964. doi: 10.13227/j.hjkx.202405157.
In order to investigate the effects of the interaction between straw return and chemical fertilizer on soil microbial activity, microbial biomass (SMB), soil microbial entropy (qmb), and soil-microbial stoichiometric imbalance in agricultural soil so as to realize the stabilization of soil and increase the production of fertilizers, the experiment was carried out in a two-factor split-area design, with the primary treatments of straw return (W1) and straw non-return (W0) and the secondary treatments of no fertilizer, nitrogen fertilizer (N) and nitrogen-phosphorus fertilizer (NP), and nitrogen-phosphorus fertilizer (NP), to investigate the characteristics of soil-microbial carbon, nitrogen, and phosphorus changes and the coupling relationship between stoichiometric characteristics, stoichiometric imbalance, and microbial entropy under different treatments. The results showed that the soil organic carbon (SOC) content of W1NP was significantly increased compared with that of W0 (<0.05), the soil total nitrogen (TN) content of W1NP was increased by 56.56% compared with that of W0, there was no significant difference in the soil TP content (>0.05), and the C/P was significantly increased in the W1NP treatment compared with that in W0 (<0.05). The soil microbial carbon (MBC), soil microbial nitrogen (MBN), and soil microbial phosphorus (MBP) contents were significantly higher (<0.05) compared to those of W0. Soil MBN content was significantly higher in all treatments of straw return compared to that of W0 (<0.05). The difference in soil MBP content between the W0NP and W1NP treatments was not significant (>0.05), but both were significantly higher than those of the other treatments (<0.05), and MBN/MBP was significantly higher than that of W0 in all treatments (<0.05). Application of phosphorus fertilizer significantly increased qmbc, qmbn, and qmbp (<0.05), and the carbon and phosphorus imbalance ratios (C/P) of the W1 and W0 treatments were not significantly different (>0.05) but were significantly higher than in the other treatments (<0.05). The nitrogen phosphorus imbalance ratio (N/P) was significantly lower (<0.05) in all treatments compared to that in W0. Correlation analysis showed that there was a significant positive correlation between MBC, MBN, and MBP (<0.05) and all were significantly negatively correlated with stoichiometric imbalance (<0.05). Redundancy analysis showed that the closest relationship was between MBC/MBP and qmbc, a significant relationship was between MBN/MBP and microbial mass entropy phosphorus, and the closest relationship was between microbial mass entropy nitrogen and MBN/MBP (<0.05). In conclusion, the straw return with the nitrogen and phosphorus fertilizer (W1NP) treatment was optimal for improving soil nutrients and changing soil stoichiometric characteristics, as well as increasing soil fertility.
为了研究秸秆还田与化肥交互作用对农田土壤微生物活性、微生物生物量(SMB)、土壤微生物熵(qmb)以及土壤-微生物化学计量失衡的影响,以实现土壤稳定并提高肥料利用率,试验采用二因素裂区设计,主处理为秸秆还田(W1)和秸秆不还田(W0),副处理为不施肥、施氮肥(N)、施氮磷肥(NP),研究不同处理下土壤微生物碳、氮、磷变化特征以及化学计量特征、化学计量失衡与微生物熵之间的耦合关系。结果表明,与W0相比,W1NP处理的土壤有机碳(SOC)含量显著增加(<0.05),W1NP处理的土壤全氮(TN)含量比W0增加了56.56%,土壤总磷(TP)含量差异不显著(>0.05),且W1NP处理的C/P比W0显著增加(<0.05)。与W0相比,土壤微生物碳(MBC)、土壤微生物氮(MBN)和土壤微生物磷(MBP)含量显著更高(<0.05)。秸秆还田的所有处理中土壤MBN含量均显著高于W0(<0.05)。W0NP和W1NP处理间土壤MBP含量差异不显著(>0.05),但均显著高于其他处理(<0.05),且所有处理的MBN/MBP均显著高于W0(<0.05)。施用磷肥显著增加了qmbc、qmbn和qmbp(<0.05),W1和W0处理的碳磷失衡比(C/P)差异不显著(>0.05),但显著高于其他处理(<0.05)。所有处理的氮磷失衡比(N/P)均显著低于W0(<0.05)。相关性分析表明,MBC、MBN和MBP之间存在显著正相关(<0.05),且均与化学计量失衡显著负相关(<0.05)。冗余分析表明,MBC/MBP与qmbc关系最密切,MBN/MBP与微生物生物量熵磷之间存在显著关系,微生物生物量熵氮与MBN/MBP关系最密切(<0.05)。综上所述,秸秆还田配施氮磷肥(W1NP)处理在改善土壤养分、改变土壤化学计量特征以及提高土壤肥力方面效果最佳。