Wu Yulong, Zhang Tianyu, Jiang Guangyi, Duan Qingxia, Zuo Chen, Shu Wei
Department of Radiochemistry, China Institute of Atomic Energy, Beijing, 102413, PR China.
School of Life Sciences and Medicine, Shandong University of Technology, Zibo 255000, P. R. China.
Anal Methods. 2025 Jun 19;17(24):4998-5005. doi: 10.1039/d5ay00578g.
Ferroptosis, an iron-dependent, lipid peroxidation-mediated type of programmed cell death, is crucial in the pathogenesis of numerous diseases. Mitochondria, central to cellular energy production, are significantly involved in ferroptosis. Sulfur dioxide (SO) is generated within both the mitochondria and cytoplasm, and its abnormal levels are linked to mitochondrial dysfunction and various diseases. To detect mitochondrial HSO in ferroptosis, we developed CMP, a long-wavelength fluorescent probe with high specificity and a rapid response. CMP utilizes a benzopyrylium cation for HSO recognition and mitochondrial targeting. It reacts with HSO Michael addition, quenching fluorescence at 622 nm, and achieves ultrasensitive detection. CMP enables real-time HSO monitoring in HeLa cells and zebrafish, and successfully detected increased mitochondrial HSO levels during Erastin-induced ferroptosis and CCCP-induced apoptosis. CMP offers a valuable tool for studying ferroptosis-related diseases.
