Department of Pharmaceutical Chemistry, School of Pharmaceutical Sciences, Guangxi Medical University, No. 22, Shuangyong Road, Nanning, Guangxi, 530021, China; Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, CAS Research/Education Center for Excellence in Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China; University of Chinese Academy of Sciences, Beijing, 100049, China.
Spectrochim Acta A Mol Biomol Spectrosc. 2022 Oct 5;278:121305. doi: 10.1016/j.saa.2022.121305. Epub 2022 Apr 27.
Sulfur dioxide, an essential gas signaling molecule mainly produced in mitochondria, plays important roles in many physiological and pathological processes. Herein, a near-infrared fluorescent probe, A1, with good mitochondria targeting ability was developed for colorimetric and fluorescence detection of HSO. Probe A1 has a conjugated cyanine structure that can selectively react with HSO through the nucleophilic addition. The reaction with HSO destroys the conjugated structure of probe A1, resulting in fluorescence quenching, and accompaniedby color change of probe A1 solution from purple-red to colorless. Probe A1 showed high selectivity and good sensitivity to HSO in PBS. And the limit of detection was calculated to be 1.28 and 0.037 μM for colorimetry and fluorescence spectrophotometry respectively. In addition, probe A1 mainly entered the mitochondria in living cells, and was successfully used for imaging the exogenous/endogenous HSO in cells. These results suggest the potential applications of probe A1 in biological systems.
二氧化硫作为一种主要在线粒体中产生的重要气体信号分子，在许多生理和病理过程中发挥着重要作用。本文开发了一种近红外荧光探针 A1，其具有良好的线粒体靶向能力，可用于比色法和荧光法检测 HSO。探针 A1 具有共轭菁结构，可通过亲核加成选择性地与 HSO 反应。与 HSO 的反应破坏了探针 A1 的共轭结构，导致荧光猝灭，并伴随着探针 A1 溶液从紫红色变为无色的颜色变化。探针 A1 在 PBS 中对 HSO 表现出高选择性和良好的灵敏度。比色法和荧光分光光度法的检测限分别计算为 1.28 和 0.037 μM。此外，探针 A1 主要进入活细胞的线粒体，并成功用于细胞中外源/内源性 HSO 的成像。这些结果表明探针 A1 在生物系统中的潜在应用。
