Jin Shue, Cai Yongrui, Li Yaxing, Wen Jing, Fu Xiaoxue, Song Ping, Lu Pengyu, Chen Anjing, Luo Zeyu, Zeng Weinan, Li Jidong, Zhou Zongke
Department of Orthopedic Surgery and Orthopedic Research Institute, West China Hospital, Sichuan University, Chengdu, 610041, China.
Analytical & Testing Center, Sichuan University, Chengdu, 610065, China.
Bioact Mater. 2025 May 13;51:211-230. doi: 10.1016/j.bioactmat.2025.05.008. eCollection 2025 Sep.
Skeletal muscle injuries caused by trauma, infections, or sports tear are common clinical diseases. Currently, the regeneration and repair of muscle tissue, which is highly heterogeneous, remains a significant challenge. Given the anisotropic structure, high strength and tensile characteristics of skeletal muscle, this study proposes a treatment strategy for muscle injury that combines materials nano-topological cues and biochemical cues. The approach aims to facilitate muscle injury repair through the use of heterogeneous nanofibers on the surface of the sandwich-like electrospun nanofibrous scaffold and macrophage phenotype transformation. Specifically, the outer layer of the sandwich-like scaffold consists of highly aligned fibers, while the middle layer is a core-shell structured random fibers containing hyaluronic acid, and the fiber matrix is composed of optimized proportions of polycaprolactone and gelatin. Mechanical testing shows that the sandwich-like scaffold combines the excellent tensile strength of the outer aligned fibers with the larger elongation at break and suture retention strength of the inner random fibers. Cell and animal experiments confirmed that the aligned fibers in the outer layers guide the cell adhesion, cytoskeleton and nuclear remodeling, and myogenic differentiation of myoblasts, and hyaluronic acid promotes both myogenic differentiation and macrophage phenotype transformation, ultimately accelerating skeletal muscle regeneration. This sandwich-like nanofibrous scaffold provides a novel cell-free, and factor-free approach for the regeneration of skeletal muscle injuries.
由创伤、感染或运动撕裂引起的骨骼肌损伤是常见的临床疾病。目前，高度异质性的肌肉组织的再生和修复仍然是一项重大挑战。鉴于骨骼肌的各向异性结构、高强度和拉伸特性，本研究提出了一种结合材料纳米拓扑线索和生化线索的肌肉损伤治疗策略。该方法旨在通过在三明治状电纺纳米纤维支架表面使用异质纳米纤维和巨噬细胞表型转化来促进肌肉损伤修复。具体而言，三明治状支架的外层由高度排列的纤维组成，而中间层是含有透明质酸的核壳结构随机纤维，纤维基质由优化比例的聚己内酯和明胶组成。力学测试表明，三明治状支架将外层排列纤维的优异拉伸强度与内层随机纤维的较大断裂伸长率和缝线保留强度结合在一起。细胞和动物实验证实，外层的排列纤维引导成肌细胞的细胞粘附、细胞骨架和核重塑以及成肌分化，透明质酸促进成肌分化和巨噬细胞表型转化，最终加速骨骼肌再生。这种三明治状纳米纤维支架为骨骼肌损伤的再生提供了一种新颖的无细胞、无因子方法。
