John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA; Department of Mechanical Engineering, University of Michigan, Ann Arbor, MI, 48109, USA.
John A. Paulson School of Engineering and Applied Sciences, Harvard University, Cambridge, MA, 02138, USA; Wyss Institute for Biologically Inspired Engineering, Harvard University, Boston, MA, 02115, USA.
Biomaterials. 2024 Sep;309:122597. doi: 10.1016/j.biomaterials.2024.122597. Epub 2024 Apr 26.
Wounds often necessitate the use of instructive biomaterials to facilitate effective healing. Yet, consistently filling the wound and retaining the material in place presents notable challenges. Here, we develop a new class of injectable tissue adhesives by leveraging the dynamic crosslinking chemistry of Schiff base reactions. These adhesives demonstrate outstanding mechanical properties, especially in regard to stretchability and self-healing capacity, and biodegradability. Furthermore, they also form robust adhesion to biological tissues. Their therapeutic potential was evaluated in a rodent model of volumetric muscle loss (VML). Ultrasound imaging confirmed that the adhesives remained within the wound site, effectively filled the void, and degraded at a rate comparable to the healing process. Histological analysis indicated that the adhesives facilitated muscle fiber and blood vessel formation, and induced anti-inflammatory macrophages. Notably, the injured muscles of mice treated with the adhesives displayed increased weight and higher force generation than the control groups. This approach to adhesive design paves the way for the next generation of medical adhesives in tissue repair.
伤口通常需要使用有指导意义的生物材料来促进有效的愈合。然而，始终如一地填充伤口并将材料固定在适当的位置仍然存在显著的挑战。在这里，我们利用席夫碱反应的动态交联化学开发了一类新型可注射组织粘合剂。这些粘合剂表现出优异的机械性能，尤其是在拉伸性和自修复能力以及生物降解性方面。此外，它们还能与生物组织形成牢固的黏附。我们在一种容积性肌肉损失（VML）的啮齿动物模型中评估了它们的治疗潜力。超声成像证实，粘合剂仍留在伤口部位，有效地填充了空隙，并以与愈合过程相当的速度降解。组织学分析表明，粘合剂促进了肌肉纤维和血管的形成，并诱导了抗炎巨噬细胞。值得注意的是，与对照组相比，接受粘合剂治疗的小鼠的受伤肌肉显示出增加的重量和更高的力量产生。这种粘合剂设计方法为组织修复中的下一代医用粘合剂铺平了道路。
