Gonadal tissue preservation technologies and culture offer opportunities to bridge knowledge between wildlife and humans.

Gonadal tissue preservation has emerged as a promising alternative mainly for the creation of wildlife biobanks and the restoration of fertility in humans. This review aims to establish bridges between the knowledge acquired from studies in humans and wild animals, highlighting challenges and advances. Progresses in in vitro culture of tissues and grafting technologies have cross-species implications, driving innovation in both areas. In humans, research is largely focused on optimizing clinical outcomes and expanding accessibility whereas in wild animals, the emphasis is on preserving genetic diversity and addressing technical challenges of noninvasive sampling and preservation. Although gonadal tissue preservation, in vitro culture, and grafting share common foundational methodologies across humans and wild animals, their application requires species-specific adaptations due to anatomical and physiological differences. Leveraging the synergies between human and wildlife research will enhance fertility preservation and biodiversity conservation efforts globally.