Yeoh Soo Ghee, Liew Yun Khoon, Lim Wei Meng, Rahman Norizah Abdul, Then Yoon Yee
School of Postgraduate Studies, IMU University, Bukit Jalil, 57000 Kuala Lumpur, Malaysia.
Department of Life Sciences, School of Pharmacy, IMU University, Bukit Jalil, 57000 Kuala Lumpur, Malaysia.
ACS Omega. 2025 May 22;10(21):21065-21076. doi: 10.1021/acsomega.4c08100. eCollection 2025 Jun 3.
An electrospun chitosan-gelatin (CS-GL) nanofiber encapsulated with the curcumin (CUR)-chrysin (CHR)-loaded copper (Cu) metal-organic framework (MOF) was successfully synthesized in this study. A high percentage of encapsulation efficiency (99.2%) of CUR and CHR in Cu-MOF-CUR-CHR was yielded. Other than the confirmation of the successful fusion of MOFs with CUR-CHR in CS-GL/Cu-MOF-CUR-CHR nanofibers using Fourier-transform infrared (FTIR) spectroscopy, the scanning electron microscopy (SEM) images depicted an average diameter in the range of 302-380 nm. The percentage of cumulative drug release of CS-GL/20% Cu-MOF-CUR-CHR nanofibers in phosphate-buffered solution at pH 7.4 after 48 h was 98.9%, which was higher than CS-GL/CUR-CHR nanofibers without Cu-MOFs. In the cytotoxicity test on Human epidermal keratinocytes cell line cells, it was noticed that 1% and 5% CS-GL/Cu-MOF-CUR-CHR nanofibers exhibited high cell survival (>90%). Through the colony counting method, the logarithm reduction values (LRVs) of CS-GL/5% Cu-MOF-CUR-CHR nanofibers were 2.69 against Staphylococcus aureus and 1.87 against Pseudomonas aeruginosa. It was noteworthy that the CS-GL/Cu-MOF-CUR-CHR nanofiber exhibited a higher antibacterial activity against Gram-positive than Gram-negative bacteria. Overall, the biocompatible CS-GL/Cu-MOF-CUR-CHR nanofibers with an effective antibacterial effect have the potential to be used as an alternative to antibiotics in antibacterial wound dressing applications.
本研究成功合成了一种静电纺丝的壳聚糖-明胶（CS-GL）纳米纤维，其包裹着负载姜黄素（CUR）-白杨素（CHR）的铜（Cu）金属有机框架（MOF）。Cu-MOF-CUR-CHR中CUR和CHR的包封率很高（99.2%）。除了使用傅里叶变换红外（FTIR）光谱证实MOF与CS-GL/Cu-MOF-CUR-CHR纳米纤维中的CUR-CHR成功融合外，扫描电子显微镜（SEM）图像显示平均直径在302-380nm范围内。CS-GL/20%Cu-MOF-CUR-CHR纳米纤维在pH 7.4的磷酸盐缓冲溶液中48小时后的累积药物释放率为98.9%，高于不含Cu-MOF的CS-GL/CUR-CHR纳米纤维。在对人表皮角质形成细胞系细胞的细胞毒性试验中，发现1%和5%的CS-GL/Cu-MOF-CUR-CHR纳米纤维表现出较高的细胞存活率（>90%）。通过菌落计数法，CS-GL/5%Cu-MOF-CUR-CHR纳米纤维对金黄色葡萄球菌的对数减少值（LRV）为2.69，对铜绿假单胞菌的对数减少值为1.87。值得注意的是，CS-GL/Cu-MOF-CUR-CHR纳米纤维对革兰氏阳性菌的抗菌活性高于革兰氏阴性菌。总体而言，具有有效抗菌作用的生物相容性CS-GL/Cu-MOF-CUR-CHR纳米纤维有潜力在抗菌伤口敷料应用中作为抗生素的替代品。
